Gastric cancer derived exosomes mediate the delivery of circRNA to promote angiogenesis by targeting miR-29a/VEGF axis in endothelial cells

Biochem Biophys Res Commun. 2021 Jun 30:560:37-44. doi: 10.1016/j.bbrc.2021.04.099. Epub 2021 May 6.

Abstract

Accumulating evidence has been found that circular RNA (circRNA) plays a critical role in the initiation and development of various diseases by modulating gene expression in the cytoplasm. However, the role of circ_0044366 (termed circ29) in gastric cancer (GC) has yet to be elusive. We detected that exosomal circ29 was confirmed to be highly expressed in GC and can significantly impair the proliferation, migration, tube formation of HUVEC by exosomal communication. Interestingly, this effect could be blocked by the effect of miR-29a. In brief, we confirmed that circ29, as a sponge of miR-29a, plays a responsible role in the occurrence and development of GC by regulating the VEGF pathway. Therefore, it may be used as a potential target for the treatment of GC.

Keywords: Angiogenesis; Exosomes; Gastric cancer; circRNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Exosomes / genetics*
  • Exosomes / ultrastructure
  • Human Umbilical Vein Endothelial Cells / physiology
  • Humans
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • MicroRNAs / metabolism*
  • MicroRNAs / physiology
  • Neovascularization, Physiologic*
  • RNA, Circular / blood
  • RNA, Circular / metabolism*
  • RNA, Circular / physiology
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / metabolism
  • Stomach Neoplasms / pathology
  • Stomach Neoplasms / ultrastructure
  • Vascular Endothelial Growth Factor A / genetics*
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • MIRN29a microRNA, human
  • MicroRNAs
  • RNA, Circular
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A