Comparative biochemical and transcriptome analyses in tomato and eggplant reveal their differential responses to Tuta absoluta infestation

Li-Min Chen; Xiao-Wei Li; Tian-Jun He; Peng-Ju Li; Yuan Liu; Shu-Xing Zhou; Quan-Cong Wu; Ting-Ting Chen; Yao-Bin Lu; You-Ming Hou

doi:10.1016/j.ygeno.2021.05.002

Comparative biochemical and transcriptome analyses in tomato and eggplant reveal their differential responses to Tuta absoluta infestation

Genomics. 2021 Jul;113(4):2108-2121. doi: 10.1016/j.ygeno.2021.05.002. Epub 2021 May 5.

Authors

Li-Min Chen¹, Xiao-Wei Li², Tian-Jun He³, Peng-Ju Li⁴, Yuan Liu⁴, Shu-Xing Zhou², Quan-Cong Wu³, Ting-Ting Chen⁵, Yao-Bin Lu⁶, You-Ming Hou⁷

Affiliations

¹ State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Biopesticide and Chemical Biology, Ministry of Education & Fujian Province Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China; Integrated Plant Protection Center, Lishui Academy of Agricultural and Forestry Sciences, 827 Liyang Stress, Lishui, Zhejiang 323000, China.
² State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
³ Integrated Plant Protection Center, Lishui Academy of Agricultural and Forestry Sciences, 827 Liyang Stress, Lishui, Zhejiang 323000, China.
⁴ State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Biopesticide and Chemical Biology, Ministry of Education & Fujian Province Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
⁵ College of Ecology, Lishui University, Lishui, Zhejiang 323000, China.
⁶ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China. Electronic address: lvyb@zaas.ac.cn.
⁷ State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Biopesticide and Chemical Biology, Ministry of Education & Fujian Province Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. Electronic address: ymhou@fafu.edu.cn.

PMID: 33964421
DOI: 10.1016/j.ygeno.2021.05.002

Abstract

Tomato is more prone to Tuta absoluta invasion and damages as compared to other host plants but the mechanism behind this preference has not been elucidated. Here, two contrasting host preference plants, tomato and eggplant, were used to investigate biochemical and transcriptomic modifications induced by T. absoluta infestation. Biochemical analysis at 0-72 h post T. absoluta infestation revealed significantly reduced concentrations of amino acid, fructose, sucrose, jasmonic acid, salicylic acid, and total phenols in tomato compared to eggplant, mainly at 48 h post T. absoluta infestation. Transcriptome analysis showed higher transcript changes in infested eggplant than tomato. Signaling genes had significant contributions to mediate plant immunity against T. absoluta, specifically genes associated with salicylic acid in eggplant. Genes from PR1b1, NPR1, NPR3, MAPKs, and ANP1 families play important roles to mitigate T. absoluta infestation. Our results will facilitate the development of control strategies against T. absoluta for sustainable tomato production.

Keywords: Biochemical; Gene expression; Plant-Pest interaction; Solanaceae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Gene Expression Profiling
Humans
Moths* / physiology
Solanum lycopersicum* / genetics
Solanum melongena* / genetics
Transcriptome