Electrophysiological, behavioural and biochemical effect of Ocimum basilicum oil and its constituents methyl chavicol and linalool on Musca domestica L

Abstract

Ocimum basilicum essential oil (EO) was evaluated for its biological effects on M. domestica. Characterization of O. basilicum EO revealed the presence of methyl chavicol (70.93%), linalool (9.34%), epi-α-cadinol (3.69 %), methyl eugenol (2.48%), γ-cadinene (1.67%), 1,8-cineole (1.30%) and (E)-β-ocimene (1.11%). The basil EO and its constituents methyl chavicol and linalool elicited a neuronal response in female adults of M. domestica. Adult female flies showed reduced preference to food source laced with basil EO and methyl chavicol. Substrates treated with EO and methyl chavicol at 0.25% resulted in an oviposition deterrence of over 80%. A large ovicidal effect was found for O. basilicum EO (EC₅₀ 9.74 mg/dm³) followed by methyl chavicol (EC₅₀ 10.67 mg/dm³) and linalool (EC₅₀ 13.57 mg/dm³). Adults exposed to EO (LD₅₀ 10.01 μg/adult) were more susceptible to contact toxicity than to methyl chavicol and linalool (LD₅₀ 13.62 μg/adult and LD₅₀ 43.12 μg/adult respectively). EO and its constituents methyl chavicol and linalool also induced the detoxifying enzymes Carboxyl esterase (Car E) and Glutathione S - transferases (GST).

Keywords: Basil oil; Detoxifying enzymes; Electrophysiology; Housefly; Ovipositional deterrence; Secondary metabolites.