Feeder-Free Human Embryonic Stem Cell Culture Under Defined Culture Conditions

Taha Bartu Hayal; Ayşegül Doğan

doi:10.1007/7651_2021_404

Feeder-Free Human Embryonic Stem Cell Culture Under Defined Culture Conditions

Methods Mol Biol. 2022:2520:25-35. doi: 10.1007/7651_2021_404.

Authors

Taha Bartu Hayal¹, Ayşegül Doğan²

Affiliations

¹ Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey.
² Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey. aysegul.dogan@yeditepe.edu.tr.

PMID: 33959914
DOI: 10.1007/7651_2021_404

Abstract

Human embryonic stem (ES) cell culture has developed over the years allowing the subtle procedures that are easy to manipulate. Feeder-free ES cell culture is an important milestone for human pluripotent stem cell research which eliminates the feeder cells. Various matrices and medium formulations have been generated for feeder-independent culture. Here we described an mTeSR™1 based feeder-independent human ES cell culture on Matrigel^® matrix. Culture, freeze/thaw, passaging and initiation of differentiation in suspension culture were described.

Keywords: Feeder-free culture; Human embryonic stem cells; Induced pluripotent stem cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Feeder Cells
Human Embryonic Stem Cells*
Humans
Pluripotent Stem Cells*