Potential biotransformation pathways and efficiencies of ciprofloxacin and norfloxacin by an activated sludge consortium

Abstract

Fluoroquinolones (FQs), such as ciprofloxacin (CIP) and norfloxacin (NOR), are types of emerging trace pollutants that have attracted great attention. In this study, an activated sludge (AS) consortium with high bio-removal capability to CIP and NOR was obtained by acclimating with CIP and NOR for 10 d. Meanwhile, a CIP- and NOR- transforming bacterial strain (S5), which is highly homologous to the 16S rRNA gene sequence of Enterobacter sp., was isolated from the acclimated AS. The bio-removal efficiency of CIP under the acclimated AS consortium was better than that under the pure culture of Enterobacter sp. S5 (93.1% vs. 89.3%), while the bio-removal efficiency of NOR under the acclimated AS consortium was lower than that under the pure culture of Enterobacter sp. S5 (83.9% vs. 89.8%). The biotransformation and bio-adsorption were two main ways to bio-remove CIP and NOR. However, the CIP and NOR biotransformation efficiencies of the acclimated AS were higher than under the pure culture of Enterobacter sp. S5, while the CIP and NOR adsorption of acclimated AS were lower than that under the pure culture of Enterobacter sp. S5. The N-acetylciprofloxacin and N-acetylnorfloxacin were the main biotransformation products of CIP and NOR. It is possible that acetyltransferase may be involved in the biotransformation process. Whether under the pure culture or AS consortium, the cytotoxicity of CIP and NOR transformation products to gram-negative bacteria was alleviated. Therefore, the acclimated AS and Enterobacter sp. S5 might provide a new strategy for removing contaminants and alleviating of FQs resistance.

Keywords: Activated sludge; Biotransformation; Consortium; Fluoroquinolones.