Microbial and chemical pollutants on the manure-crops pathway in the perspective of "One Health" holistic approach

Martyna Buta; Ewa Korzeniewska; Monika Harnisz; Jakub Hubeny; Wiktor Zieliński; Damian Rolbiecki; Sylwia Bajkacz; Ewa Felis; Klaudia Kokoszka

doi:10.1016/j.scitotenv.2021.147411

Microbial and chemical pollutants on the manure-crops pathway in the perspective of "One Health" holistic approach

Sci Total Environ. 2021 Sep 1:785:147411. doi: 10.1016/j.scitotenv.2021.147411. Epub 2021 Apr 29.

Authors

Martyna Buta¹, Ewa Korzeniewska², Monika Harnisz¹, Jakub Hubeny¹, Wiktor Zieliński¹, Damian Rolbiecki¹, Sylwia Bajkacz³, Ewa Felis⁴, Klaudia Kokoszka³

Affiliations

¹ Department of Water Protection Engineering and Environmental Microbiology, The Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland.
² Department of Water Protection Engineering and Environmental Microbiology, The Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland. Electronic address: ewa.korzeniewska@uwm.edu.pl.
³ The Biotechnology Centre, Silesian University of Technology, Krzywoustego 8 Str., 44-100 Gliwice, Poland; Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100 Gliwice, Poland.
⁴ The Biotechnology Centre, Silesian University of Technology, Krzywoustego 8 Str., 44-100 Gliwice, Poland; Environmental Biotechnology Department, Faculty of Energy and Environmental Engineering, Silesian University of Technology, Akademicka 2 Str., 44-100 Gliwice, Poland.

PMID: 33957582
DOI: 10.1016/j.scitotenv.2021.147411

Abstract

This study determined the impact of poultry and bovine manure fertilization on the content of antibiotics, heavy metals (HMs), and the quantitative and qualitative composition of integrase and antibiotic resistance genes in soil, groundwater, and crops cultivated on manure-amended plots. Antibiotic concentration levels were analyzed using the HPLC-MS/MS, heavy metal concentration level were measured by HGAAS and ICP-OES, while the integrase genes and ARGs were quantified using Real-Time PCR (qPCR) method. Manure, soil, and crops samples contained the highest concentration of Zn (10⁴-10⁵ ng g_dm^-1) and Cu (10³-10⁵ ng g_dm^-1) of all HMs tested. Manure-supplemented soil was characterised by a high concentration of doxycycline and enrofloxacin. A high abundance of integrase genes was noted in samples of manure (10⁹-10¹⁰ copies g_dm^-1) and soil (10⁷-10⁸ copies g_dm^-1). Among all the analyzed genes, sul1, sul2, bla_TEM, and integrase genes were the most common. Results of the study demonstrate the selective character of ARGs transfer from poultry and bovine manure to plants. The only gene to occur in all studied environmental compartments was sul1 (from 10² - groundwater to 10¹¹ - poultry manure). It was also found that animal manure may cause an increase in the HMs concentration in soil and their accumulation in crops, which may influence the health of humans and animals consuming crops grown on manure-amended soil. The high abundance of integrase genes and ARGs and their reciprocal correlations with HMs pose a serious risk of the rapid spread of antibiotic resistance in the environment. Moreover, unusual dependencies between integrase genes and selected ARGs indicate the possibility of changes in the mobility nature of genetic elements.

Keywords: Antibiotic resistance genes; Antibiotics; Heavy metals; Integrase genes; Manure; Soil.

MeSH terms

Animals
Anti-Bacterial Agents
Cattle
Environmental Pollutants*
Genes, Bacterial
Humans
Manure*
Soil
Soil Microbiology
Tandem Mass Spectrometry

Substances

Anti-Bacterial Agents
Environmental Pollutants
Manure
Soil