Abstract
We report a facile and reversible method to immobilize a broad range of His6-tagged proteins on the E. coli cell surface through Fe(iii)-metal complexes. A His6-tagged eGFP and four His6-tagged enzymes were successfully immobilized on the cell surface. Additionally, a hydrogel sheath around E. coli cells was generated by immobilized His6-tagged HRP.
MeSH terms
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Alcohol Oxidoreductases / chemistry
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Alcohol Oxidoreductases / metabolism*
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Bacillus licheniformis / enzymology
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Bacillus subtilis / enzymology
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Candida tropicalis / enzymology
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Cell Membrane / chemistry
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Cell Membrane / metabolism
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Escherichia coli / chemistry
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Escherichia coli / cytology
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Escherichia coli / metabolism*
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Ferric Compounds / chemistry
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Ferric Compounds / metabolism*
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Green Fluorescent Proteins / chemistry
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Green Fluorescent Proteins / metabolism*
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Histidine / chemistry
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Histidine / metabolism
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Laccase / chemistry
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Laccase / metabolism*
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Lipase / chemistry
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Lipase / metabolism*
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Oligopeptides / chemistry
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Oligopeptides / metabolism
Substances
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Ferric Compounds
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His-His-His-His-His-His
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Oligopeptides
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Green Fluorescent Proteins
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Histidine
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Alcohol Oxidoreductases
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Laccase
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Lipase