Fe(III)-complex mediated bacterial cell surface immobilization of eGFP and enzymes

Chem Commun (Camb). 2021 May 4;57(36):4460-4463. doi: 10.1039/d1cc01575c.

Abstract

We report a facile and reversible method to immobilize a broad range of His6-tagged proteins on the E. coli cell surface through Fe(iii)-metal complexes. A His6-tagged eGFP and four His6-tagged enzymes were successfully immobilized on the cell surface. Additionally, a hydrogel sheath around E. coli cells was generated by immobilized His6-tagged HRP.

MeSH terms

  • Alcohol Oxidoreductases / chemistry
  • Alcohol Oxidoreductases / metabolism*
  • Bacillus licheniformis / enzymology
  • Bacillus subtilis / enzymology
  • Candida tropicalis / enzymology
  • Cell Membrane / chemistry
  • Cell Membrane / metabolism
  • Escherichia coli / chemistry
  • Escherichia coli / cytology
  • Escherichia coli / metabolism*
  • Ferric Compounds / chemistry
  • Ferric Compounds / metabolism*
  • Green Fluorescent Proteins / chemistry
  • Green Fluorescent Proteins / metabolism*
  • Histidine / chemistry
  • Histidine / metabolism
  • Laccase / chemistry
  • Laccase / metabolism*
  • Lipase / chemistry
  • Lipase / metabolism*
  • Oligopeptides / chemistry
  • Oligopeptides / metabolism

Substances

  • Ferric Compounds
  • His-His-His-His-His-His
  • Oligopeptides
  • Green Fluorescent Proteins
  • Histidine
  • Alcohol Oxidoreductases
  • Laccase
  • Lipase