The research on polyethylene terephthalate (PET) hydrolyzing enzymes started in 2005; several studies are now nearing the objective of their application in biorecycling of PET, which is an urgent environmental issue. The thermostability of PET hydrolases must be higher than 70 °C, which has already been established by several thermophilic cutinases, as higher thermostability results in higher activity. Additionally, pretreatment of waste PET to more enzyme-attackable forms is necessary for PET biorecycling. This Minireview summarizes research on enzymatic PET hydrolysis from two viewpoints: 1) improvement of PET hydrolases by focusing on their thermostabilities by mutation of enzyme genes, their expression in several hosts, and their modifications; and 2) processing of waste PET to readily biodegradable forms. Finally, the outlook of PET biorecycling is described.
Keywords: PET hydrolase; biorecycling; cutinases; polyethylene terephthalate; waste plastics.
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