A novel and injectable strontium-containing hydroxyapatite bone cement for bone substitution: A systematic evaluation

Jiewen Dai; Yuanfei Fu; Demin Chen; Zhaoyao Sun

doi:10.1016/j.msec.2021.112052

A novel and injectable strontium-containing hydroxyapatite bone cement for bone substitution: A systematic evaluation

Mater Sci Eng C Mater Biol Appl. 2021 May:124:112052. doi: 10.1016/j.msec.2021.112052. Epub 2021 Mar 25.

Authors

Jiewen Dai¹, Yuanfei Fu², Demin Chen³, Zhaoyao Sun⁴

Affiliations

¹ Department of Oral & Cranio-Maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China. Electronic address: daijiewen@163.com.
² Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China. Electronic address: fuyuanf@163.com.
³ Biomaterials Research and Test Center, Shanghai Ninth People's Hospital, Shanghai JiaoTong University, School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China. Electronic address: chendemin@smmail.cn.
⁴ Biomaterials Research and Test Center, Shanghai Ninth People's Hospital, Shanghai JiaoTong University, School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China. Electronic address: sunzy013@163.com.

PMID: 33947546
DOI: 10.1016/j.msec.2021.112052

Abstract

Reconstruction of bone defects is still a challenge. In this study, we developed and systematically evaluated a novel injectable strontium-containing hydroxyapatite (Sr-HA) bone cement in which Sr-HA powder included 5% Sr and was mixed with a setting liquid that included 5% potassium citrate. This Sr-HA cement was mainly composed of HA and α-tricalcium phosphate (TCP) and exhibited favorable injectability (100%), setting times (the initial setting time was 240 s and the final setting time was 420 s), compressive strength (73.4 MPa), maximal load and maximum bending stress, and excellent radiopacity. In addition, the Sr-HA cement also had excellent biocompatibility that exhibited low cytotoxicity for cell proliferation and no obvious disturbing effect on the osteogenic differentiation of periodontal ligament stem cells (DLSCs) and dental pulp stem cells (DPSCs). However, the Sr-HA cement could slightly promote the osteogenic differentiation of MC3T3 cells, which also implied that it would promote osseointegration between the cement and surrounding bone but would not obviously disturb the biological behavior of DLSCs and DPSCs. An in vivo study further confirmed that Sr-HA cement exhibited favorable osseointegration with the maxilla and tibia. All these findings implied that the novel Sr-HA cement was a suitable bone substitution for bone defects.

Keywords: Bone substitution; Hydroxyapatite bone cement; Injectable; Strontium.

MeSH terms

Bone Cements* / pharmacology
Calcium Phosphates
Durapatite
Materials Testing
Osteogenesis
Strontium* / pharmacology

Substances

Bone Cements
Calcium Phosphates
strontium-containing hydroxyapatite
Durapatite
Strontium