Microbial community response to ciprofloxacin toxicity in sponge membrane bioreactor

Bao-Trong Dang; Xuan-Thanh Bui; Tomoaki Itayama; Huu Hao Ngo; Deokjin Jahng; Chitsan Lin; Shiao-Shing Chen; Kun-Yi Andrew Lin; Thanh-Tin Nguyen; Dinh Duc Nguyen; Todd Saunders

doi:10.1016/j.scitotenv.2021.145041

Microbial community response to ciprofloxacin toxicity in sponge membrane bioreactor

Sci Total Environ. 2021 Jun 15:773:145041. doi: 10.1016/j.scitotenv.2021.145041. Epub 2021 Feb 7.

Authors

Affiliations

¹ Graduate School of Engineering, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan; Ho Chi Minh City University of Technology (HUTECH) 475A, Dien Bien Phu, Ward 25, Binh Thanh District, Ho Chi Minh City, Viet Nam.
² Key Laboratory of Advanced Waste Treatment Technology, Vietnam National University Ho Chi Minh (VNU-HCM), Linh Trung ward, Thu Duc district, Viet Nam; Faculty of Environment and Natural Resources, Ho Chi Minh City University of Technology (HCMUT), Ho Chi Minh City 700000, Viet Nam. Electronic address: bxthanh@hcmut.edu.vn.
³ Graduate School of Engineering, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. Electronic address: itayama@nagasaki-u.ac.jp.
⁴ Center for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Australia.
⁵ Department of Environmental Engineering and Energy, Myongji University, Republic of Korea.
⁶ College of Maritime, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan. Electronic address: ctlin@nkust.edu.tw.
⁷ Institute of Environmental Engineering and Management, National Taipei University of Technology, Taipei, Taiwan.
⁸ Department of Environmental Engineering, National Chung Hsing University, No. 250 Kuo-Kuang Road, Taichung 402, Taiwan.
⁹ Institute of Research and Development, Duy Tan University, 03 Quang Trung, Da Nang, Viet Nam.
¹⁰ Department of Environmental Energy Engineering, Kyonggi University, 442-760, Republic of Korea.
¹¹ Graduate School of Biomedical Science, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.

PMID: 33940712
DOI: 10.1016/j.scitotenv.2021.145041

Abstract

This study aims to offer insights into how ciprofloxacin (CIP) impact bacterial community structures in the Sponge-MBR process when CIP is spiked into hospital wastewater. We found that the CIP toxicity decreased richness critical phylotypes such as phylum class ẟ-, β-, ɣ-proteobacteria, and Flavobacteria that co-respond to suppress denitrification and cake fouling to 37% and 28% respectively. Cluster analysis shows that the different community structures were formed under the influence of CIP toxicity. CIP decreased attached growth biomass by 2.3 times while increasing the concentration of permeate nitrate by 3.8 times, greatly affecting TN removal by up to 26%. Ammonia removal was kept stable by inflating the ammonia removal rate (p < 0.003), with the wealthy Nitrospira genus guaranteeing the nitrification activity. In addition, we observed an increasing richness of Chloroflexi and Planctomycetes, which may play a role in fouling reduction in the Sponge-MBR. Therefore, if the amount of antibiotics in hospital wastewater continues to increase, it is so important to extend biomass retention for denitrification recovery.

Keywords: Antibiotics; Denitrification; Hospital wastewater; Membrane fouling; Microbial community; Sponge membrane bioreactor.

MeSH terms

Bioreactors
Ciprofloxacin* / toxicity
Microbiota*
Nitrification
Wastewater

Substances

Waste Water
Ciprofloxacin