The genome of the euryhaline rotifer Brachionus paranguensis: Potential use in molecular ecotoxicology

Comp Biochem Physiol Part D Genomics Proteomics. 2021 Sep:39:100836. doi: 10.1016/j.cbd.2021.100836. Epub 2021 Apr 18.

Abstract

Brachionus spp. rotifers have been proposed as model organisms for ecotoxicological studies. We analyzed the whole-genome sequence of B. paranguensis through NextDenovo, resulting in a total length of 106.2 Mb and 71 contigs. The N50 and the GC content were 4.13 Mb and 28%, respectively. A total of 18,501 genes were predicted within the genome of B. paranguensis. Prominent detoxification-related gene families of phase I and II detoxifications have been investigated. In parallel with other Brachionus rotifers, high gene expansion was observed in CYP clan 3 and GST sigma class in B. paranguensis. Moreover, species-specific expansion of sulfotransferase (SULTs) and gain of UDP-glucuronosyltransferases (UGTs) through horizontal gene transfer has been specifically found within B. plicatilis complex. This whole-genome analysis of B. paranguensis provides a basis for molecular ecotoxicological studies and provides useful information for comparative studies of the evolution of detoxification mechanisms in Brachionus spp.

Keywords: Brachionus paranguensis; Detoxification gene; Genome; Rotifer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ecotoxicology*
  • Gene Expression Regulation*
  • Genome, Helminth*
  • Helminth Proteins / genetics
  • Helminth Proteins / metabolism*
  • Metagenomics*
  • Phylogeny
  • Rotifera / genetics*
  • Species Specificity
  • Water Pollutants, Chemical / toxicity*

Substances

  • Helminth Proteins
  • Water Pollutants, Chemical