An intensive and glow-type chemiluminescence of luminol-embedded, guanosine-derived hydrogel

Jing Ye; Liping Zhu; Mengxia Yan; Ting Xiao; Libing Fan; Yu Xue; Jianshe Huang; Xiurong Yang

doi:10.1016/j.talanta.2021.122351

An intensive and glow-type chemiluminescence of luminol-embedded, guanosine-derived hydrogel

Talanta. 2021 Aug 1:230:122351. doi: 10.1016/j.talanta.2021.122351. Epub 2021 Mar 23.

Authors

Jing Ye¹, Liping Zhu¹, Mengxia Yan², Ting Xiao², Libing Fan³, Yu Xue², Jianshe Huang⁴, Xiurong Yang⁵

Affiliations

¹ Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China.
² Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, 130022, China.
³ Department of Chemistry, Jilin University, Changchun, 130023, China.
⁴ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, 130022, China. Electronic address: huangjs@ciac.ac.cn.
⁵ Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, 130022, China. Electronic address: xryang@ciac.ac.cn.

PMID: 33934799
DOI: 10.1016/j.talanta.2021.122351

Abstract

In this paper, an intensive and glow-type chemiluminescence (CL) hydrogel was prepared by simultaneous incorporation of chemiluminescence reagent (luminol) and catalytic cofactor (hemin) into the scaffold of guanosine-derived hydrogel. The self-assembled hydrogel consisted of K⁺ stabilized hemin/G-quartet structures, showing significant enzyme-like activity to H₂O₂-mediated oxidation of luminol. After adding H₂O₂ into the hydrogel, blue light visible to naked eyes would come into being and last for over 8 h. The lasting-time CL emission of hydrogel was achieved due to a mechanism of slow-diffusion-controlled heterogeneous catalysis. Moreover, this self-assembled hydrogel performed a good response to H₂O₂ and the CL emission images could be recorded by smartphone. The hydrogel could remain excellent lifetime stability for months and the stable, enhanced and glow-type CL emission could improve the reliability and precision of CL detection, which has a promising application in cold light source and H₂O₂ detection of real biological samples.

Keywords: Chemiluminescence; Glow-type; Guanosine; Hydrogel; Luminol.