Abstract
The anti-idiotypic antibody is widely used in the field of immunology to simulate structural features or even induce the biological activity of antigens. In this study, we obtained seven anti-idiotypic single-chain variable fragments (scFv) antibodies of Cry2Aa toxin from a phage-displayed mutant library constructed using error-prone PCR technique. A mutant designated 2-12B showed the best binding ability amongst all anti-idiotypic scFv isolates to Plutella xylostella brush border membrane vesicles (BBMVs). 2-12B and Cry2Aa toxin shared a potential receptor of polycalin in P. xylostella BBMVs. Homology modeling and molecular docking demonstrated that 2-12B and Cry2Aa toxin have seven common binding amino acid residues in polycalin. Insect bioassay results suggested that 2-12 had insecticidal efficacy against P. xylostella larvae. These results indicated that the Cry2Aa anti-idiotypic scFv antibody 2-12B partially mimicked the structure and function of Cry2Aa toxin. The anti-idiotypic scFv antibody provides the basic material for the future study of surrogate molecules or new insecticidal materials.
Keywords:
Anti-idiotypic antibody; Cry2Aa toxin; Single-chain variable fragment (scFv).
Copyright © 2021 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Anti-Idiotypic / chemistry*
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Antibodies, Anti-Idiotypic / genetics
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Antibodies, Anti-Idiotypic / immunology
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Antibodies, Anti-Idiotypic / metabolism
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Antibodies, Monoclonal / chemistry*
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Antibodies, Monoclonal / genetics
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / metabolism
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Bacillus thuringiensis Toxins / chemistry*
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Bacillus thuringiensis Toxins / immunology
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Bacillus thuringiensis Toxins / metabolism
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Cell Membrane / metabolism
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Endotoxins / chemistry*
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Endotoxins / immunology
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Endotoxins / metabolism
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Hemolysin Proteins / chemistry*
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Hemolysin Proteins / immunology
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Hemolysin Proteins / metabolism
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Immunoglobulin Variable Region / chemistry*
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Immunoglobulin Variable Region / genetics
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Immunoglobulin Variable Region / immunology
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Immunoglobulin Variable Region / metabolism
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Molecular Docking Simulation
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Moths
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Mutation
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Peptide Library
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Protein Conformation
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Single-Chain Antibodies / chemistry*
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Single-Chain Antibodies / genetics
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Single-Chain Antibodies / immunology
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Single-Chain Antibodies / metabolism
Substances
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Antibodies, Anti-Idiotypic
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Antibodies, Monoclonal
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Bacillus thuringiensis Toxins
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Endotoxins
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Hemolysin Proteins
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Immunoglobulin Variable Region
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Peptide Library
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Single-Chain Antibodies
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insecticidal crystal protein, Bacillus Thuringiensis