T-cell receptor (TCR)-Vβ repertoire analysis is a sensitive method for detection of T-cell clonality. This type of analysis has been used for studying selective T-cell responses in autoimmune disease, alloreactivity in transplantation, and protective immunity against microbial and tumor antigens and in neoplastic T cells. Here, we describe the flow cytometric methods to perform this analysis.
Keywords: Clonality; Clonogram; Flow cytometry; Monoclonal antibodies; Repertoire; T-cell receptor (TCR)-Vβ.