Closed and High-Quality Bacterial Genome Sequences of the Oligo-Mouse-Microbiota Community

Quentin Lamy-Besnier; Romain Koszul; Laurent Debarbieux; Martial Marbouty

doi:10.1128/MRA.01396-20

Closed and High-Quality Bacterial Genome Sequences of the Oligo-Mouse-Microbiota Community

Microbiol Resour Announc. 2021 Apr 29;10(17):e01396-20. doi: 10.1128/MRA.01396-20.

Authors

Quentin Lamy-Besnier^{1

2

3}, Romain Koszul², Laurent Debarbieux⁴, Martial Marbouty⁵

Affiliations

¹ Bacteriophage, Bacterium, Host Laboratory, Department of Microbiology, Institut Pasteur, Paris, France.
² Unité Régulation Spatiale des Génomes, Institut Pasteur, CNRS, UMR 3525, Paris, France.
³ Université Paris Descartes, Paris, France.
⁴ Bacteriophage, Bacterium, Host Laboratory, Department of Microbiology, Institut Pasteur, Paris, France laurent.debarbieux@pasteur.fr martial.marbouty@pasteur.fr.
⁵ Unité Régulation Spatiale des Génomes, Institut Pasteur, CNRS, UMR 3525, Paris, France laurent.debarbieux@pasteur.fr martial.marbouty@pasteur.fr.

Abstract

The Oligo-Mouse-Microbiota (OMM¹²) gnotobiotic murine model is an increasingly popular model in microbiota studies. However, following Illumina and PacBio sequencing, the genomes of the 12 strains could not be closed. Here, we used genomic chromosome conformation capture (Hi-C) data to reorganize, close, and improve the quality of these 12 genomes.