Structural characterization of Alpiniae oxyphyllae fructus polysaccharide 2 and its activation effects on RAW264.7 macrophages

Xin Yang; Shiwei Zhou; Hong Li; Jiangwen An; Chengheng Li; Ruigang Zhou; Ling Teng; Yongjian Zhu; Suya Liao; Yuhui Yang; Huricha Chen; Yun Chen

doi:10.1016/j.intimp.2021.107708

Structural characterization of Alpiniae oxyphyllae fructus polysaccharide 2 and its activation effects on RAW264.7 macrophages

Int Immunopharmacol. 2021 Aug:97:107708. doi: 10.1016/j.intimp.2021.107708. Epub 2021 Apr 27.

Authors

Xin Yang¹, Shiwei Zhou¹, Hong Li¹, Jiangwen An¹, Chengheng Li¹, Ruigang Zhou¹, Ling Teng¹, Yongjian Zhu¹, Suya Liao¹, Yuhui Yang¹, Huricha Chen¹, Yun Chen²

Affiliations

¹ Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.
² Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China. Electronic address: ychen@hainanu.edu.cn.

PMID: 33915496
DOI: 10.1016/j.intimp.2021.107708

Abstract

Polysaccharides are important components of Alpiniae oxyphyllae fructus that have been shown to exhibit significant immunomodulatory activity in our previous study. However, whether and how A. oxyphyllae fructus polysaccharides (AOFP) affect macrophages has not been determined. To further study the immunomodulatory activity of AOFP, the effect of AOFP on RAW264.7 cell activation was investigated in the present work. The results showed that AOFP2 significantly increased the phagocytic activity of RAW264.7 macrophages. AOFP2 promoted the secretion of TNF-α, IL-6, IL-10, TGF-β, NO and iNOS and enhanced the Th2-type immune response via its activation effect on macrophages. Additionally, the structure of AOFP2 was characterized in the present study, as the structural features of polysaccharides determine their biological activities. AOFP2 was only composed of glucose, exhibiting an average molecular weight of 44.3 kDa. Furthermore, the infrared spectroscopy, methylation and nuclear magnetic resonance results indicated that AOFP2 consisted of → 4)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1 → and T-α-Glcp.

Keywords: Alpiniae oxyphyllae fructus polysaccharides; Macrophages; Structural characterization.

MeSH terms

Alpinia / chemistry*
Animals
Immunologic Factors / chemistry
Immunologic Factors / pharmacology*
Macrophages / drug effects*
Mice
Molecular Weight
Plant Extracts / chemistry*
Polysaccharides / chemistry
Polysaccharides / pharmacology*
RAW 264.7 Cells

Substances

Alpinia oxyphylla fruit extract
Immunologic Factors
Plant Extracts
Polysaccharides