A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system

STAR Protoc. 2021 Apr 10;2(2):100445. doi: 10.1016/j.xpro.2021.100445. eCollection 2021 Jun 18.

Abstract

Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establish an in vivo liver tumorigenesis model. By using this model, the impact of genes of interest in liver tumorigenesis and progression can be studied. This model can also be applied to develop new therapeutic drugs for HCC. For complete details on the use and execution of this protocol, please refer to He et al. (2020).

Keywords: Cancer; Gene Expression; Genetics; Model Organisms; Molecular Biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology
  • Carcinogenesis / drug effects
  • Carcinogenesis / genetics*
  • Carcinogenesis / pathology
  • DNA Transposable Elements / genetics*
  • Female
  • Genetic Engineering / methods*
  • HEK293 Cells
  • Hepatocytes / cytology
  • Hepatocytes / drug effects
  • Humans
  • Liver / drug effects
  • Liver / metabolism
  • Liver / pathology
  • Liver Neoplasms* / genetics
  • Liver Neoplasms* / metabolism
  • Liver Neoplasms* / pathology
  • Male
  • Mice
  • Models, Biological

Substances

  • Antineoplastic Agents
  • DNA Transposable Elements