Daytime Restricted Feeding Affects Day-Night Variations in Mouse Cerebellar Proteome

Fabrice Bertile; Marine Plumel; Pauline Maes; Aurélie Hirschler; Etienne Challet

doi:10.3389/fnmol.2021.613161

Daytime Restricted Feeding Affects Day-Night Variations in Mouse Cerebellar Proteome

Front Mol Neurosci. 2021 Apr 12:14:613161. doi: 10.3389/fnmol.2021.613161. eCollection 2021.

Authors

Fabrice Bertile¹, Marine Plumel¹, Pauline Maes¹, Aurélie Hirschler¹, Etienne Challet²

Affiliations

¹ Institut Pluridisciplinaire Hubert Curien, LSMBO, Université de Strasbourg, Centre National de la Recherche Scientifique, Strasbourg, France.
² Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Centre National de la Recherche Scientifique, Strasbourg, France.

Abstract

The cerebellum harbors a circadian clock that can be shifted by scheduled mealtime and participates in behavioral anticipation of food access. Large-scale two-dimensional difference gel electrophoresis (2D-DIGE) combined with mass spectrometry was used to identify day-night variations in the cerebellar proteome of mice fed either during daytime or nighttime. Experimental conditions led to modified expression of 89 cerebellar proteins contained in 63 protein spots. Five and 33 spots were changed respectively by time-of-day or feeding conditions. Strikingly, several proteins of the heat-shock protein family (i.e., Hsp90aa1, 90ab1, 90b1, and Hspa2, 4, 5, 8, 9) were down-regulated in the cerebellum of daytime food-restricted mice. This was also the case for brain fatty acid protein (Fabp7) and enzymes involved in oxidative phosphorylation (Ndufs1) or folate metabolism (Aldh1l1). In contrast, aldolase C (Aldoc or zebrin II) and pyruvate carboxylase (Pc), two enzymes involved in carbohydrate metabolism, and vesicle-fusing ATPase (Nsf) were up-regulated during daytime restricted feeding, possibly reflecting increased neuronal activity. Significant feeding × time-of-day interactions were found for changes in the intensity of 20 spots. Guanine nucleotide-binding protein G(o) subunit alpha (Gnao1) was more expressed in the cerebellum before food access. Neuronal calcium-sensor proteins [i.e., parvalbumin (Pvalb) and visinin-like protein 1 (Vsnl1)] were inversely regulated in daytime food-restricted mice, compared to control mice fed at night. Furthermore, expression of three enzymes modulating the circadian clockwork, namely heterogeneous nuclear ribonucleoprotein K (Hnrnpk), serine/threonine-protein phosphatases 1 (Ppp1cc and Ppp1cb subunits) and 5 (Ppp5), was differentially altered by daytime restricted feeding. Besides cerebellar proteins affected only by feeding conditions or daily cues, specific changes in in protein abundance before food access may be related to behavioral anticipation of food access and/or feeding-induced shift of the cerebellar clockwork.

Keywords: cerebellum; circadian clock; circadian rhythm; food restriction; neuroproteomics; proteomics.