Species-specific identification of porcine blood plasma in heat-treated chicken meatballs

Safiyyah Shahimi; Sahilah Abd Mutalib; Norfadzilah Ismail; Aishah Elias; Haslaniza Hashim; Mohd Izhar Ariff Mohd Kashim

doi:10.1016/j.sjbs.2021.01.043

Species-specific identification of porcine blood plasma in heat-treated chicken meatballs

Saudi J Biol Sci. 2021 Apr;28(4):2447-2452. doi: 10.1016/j.sjbs.2021.01.043. Epub 2021 Feb 13.

Authors

Safiyyah Shahimi^{1

2}, Sahilah Abd Mutalib^{1

3}, Norfadzilah Ismail¹, Aishah Elias¹, Haslaniza Hashim¹, Mohd Izhar Ariff Mohd Kashim⁴

Affiliations

¹ Department of Food Science, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43500 UKM Bangi, Selangor, Malaysia.
² Universiti Teknologi MARA, Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000 Kuala Pilah, Malaysia.
³ Innovation Centre for Confectionery Technology (MANIS), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.
⁴ Center of Shariah, Faculty of Islamic Studies, Universiti Kebangsaan Malaysia, 43500 UKM Bangi, Malaysia.

Abstract

This study was conducted to detect the presence of chicken and porcine DNA in meatballs using mitochondria DNA (mtDNA) of cytochrome b (cyt b) and nuclear DNA (nDNA) short interspersed nuclear element (SINE) species-specific primers, respectively. While, the mtDNA primers targeted transfer RNA-ATP8 (tRNA-ATP8) gene was used for 1 and 5% (w/w) chicken meatball spiked with commercial porcine blood plasm. Chicken meatballs spiked with 1% and 5% (v/w) fresh and commercial porcine blood plasma, respectively were prepared and heat-treated using five (n = 5) cooking methods: boiling, pan-frying, roasting, microwaving and autoclaving. Two pairs of mtDNA and nDNA primers used, produced 129 and 161 bp amplicons, respectively. Whereas, tRNA-ATP8 primers produced 212 bp of amplicon. Electrophoresis analysis showed positive results for porcine DNA at 1% and 5% (w/w or v/v) for all of the different cooking techniques, either for fresh or commercial blood plasma using SINE primers but not for tRNA-ATP8 primers. The present study has highlighted the useful of species-specific primers of SINE primers in PCR analysis for detecting porcine DNA blood plasma in heat-treated chicken meatballs.

Keywords: Chicken meatballs; DNA animal species; Polymerase chain reaction (PCR) analysis; Porcine blood plasma.