A novel approach for efficient fabrication of chitosan nanoparticles-embedded bacterial nanocellulose conduits

Zhao Wei; Pan Pan; Feng F Hong; Zhangjun Cao; Ying Ji; Lin Chen

doi:10.1016/j.carbpol.2021.118002

A novel approach for efficient fabrication of chitosan nanoparticles-embedded bacterial nanocellulose conduits

Carbohydr Polym. 2021 Jul 15:264:118002. doi: 10.1016/j.carbpol.2021.118002. Epub 2021 Mar 27.

Authors

Zhao Wei¹, Pan Pan¹, Feng F Hong², Zhangjun Cao³, Ying Ji⁴, Lin Chen⁵

Affiliations

¹ College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, No. 2999 North Renmin Road, Shanghai, 201620, China; Scientific Research Base of Bacterial Nanofiber Manufacturing and Composite Technology, China Textile Engineering Society, China.
² College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, No. 2999 North Renmin Road, Shanghai, 201620, China; State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China; Scientific Research Base of Bacterial Nanofiber Manufacturing and Composite Technology, China Textile Engineering Society, China. Electronic address: fhong@dhu.edu.cn.
³ College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, No. 2999 North Renmin Road, Shanghai, 201620, China; State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China; Scientific Research Base of Bacterial Nanofiber Manufacturing and Composite Technology, China Textile Engineering Society, China.
⁴ Institute of Textiles and Clothing, Hong Kong Polytechnic University, Hunghom, Kowloon, Hong Kong.
⁵ College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, No. 2999 North Renmin Road, Shanghai, 201620, China; Scientific Research Base of Bacterial Nanofiber Manufacturing and Composite Technology, China Textile Engineering Society, China. Electronic address: lchen@dhu.edu.cn.

PMID: 33910735
DOI: 10.1016/j.carbpol.2021.118002

Abstract

Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material. However, the conventional strategies result in poor composite effect with low efficiency. In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites. This composite method is simple and efficient, without introducing dispersants and crosslinking agents, while retaining the mechanical properties and native 3D network structure of BNC. The CSNPs-BNC composites had excellent antibacterial activity to support potential clinical application. The CSNPs-BNC composites could promote the adhesion and proliferation of Schwann cells, and demonstrate good biocompatibility both in vitro and in vivo. The results indicated that CSNPs-BNC can provide a promising candidate for biomedical applications.

Keywords: Antibacterial activity; Bacterial nanocellulose; Chitosan nanoparticles; Composite; Cytocompatibility; Dispersant; Ionic gelation; Template effect.

MeSH terms

Animals
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Bacteria / chemistry*
Cell Adhesion / drug effects
Cell Proliferation / drug effects
Cellulose / chemistry*
Cellulose / pharmacology
Chitosan / chemistry*
Chitosan / pharmacology
Elastic Modulus
Nanocomposites / chemistry
Nanoparticles / chemistry*
Particle Size
Prostheses and Implants
Rats
Schwann Cells
Tensile Strength

Substances

Anti-Bacterial Agents
Cellulose
Chitosan