AMPA Receptors Exist in Tunable Mobile and Immobile Synaptic Fractions In Vivo

Haiwen Chen; Richard H Roth; Elena Lopez-Ortega; Han L Tan; Richard L Huganir

doi:10.1523/ENEURO.0015-21.2021

AMPA Receptors Exist in Tunable Mobile and Immobile Synaptic Fractions In Vivo

eNeuro. 2021 May 21;8(3):ENEURO.0015-21.2021. doi: 10.1523/ENEURO.0015-21.2021. Print 2021 May-Jun.

Authors

Haiwen Chen^{1

2

3}, Richard H Roth³, Elena Lopez-Ortega³, Han L Tan³, Richard L Huganir⁴

Affiliations

¹ Division of Pediatric Neurology, Department of Neurology, Johns Hopkins University School of Medicine, MD 21205.
² Department of Pediatrics, Johns Hopkins University School of Medicine, MD 21205.
³ Solomon H. Snyder Department of Neuroscience and Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
⁴ Solomon H. Snyder Department of Neuroscience and Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205 rhuganir@jhmi.edu.

Abstract

AMPA receptor (AMPAR) mobility within synapses has been extensively studied in vitro However, whether similar mobility properties apply to AMPARs in vivo has yet to be determined. Here, we use two-photon fluorescence recovery after photobleaching (FRAP) to study AMPAR mobility within individual dendritic spines in live animals using an overexpression vector. We demonstrate the existence of mobile and immobile fractions of AMPARs across multiple cortical regions and layers. Additionally, we find that AMPAR mobility can be altered in vivo in response to administration of corticosterone, a condition that mimics exposure to stress.

Keywords: GluA1; plasticity; stress; synapses.

Publication types

Review

MeSH terms

Animals
Corticosterone
Neuronal Plasticity*
Receptors, AMPA*
Synapses

Substances

Receptors, AMPA
Corticosterone

Abstract

Publication types

MeSH terms

Substances

Grants and funding