Effects of mixed antimicrobial peptide on the growth performance, antioxidant and immune responses and disease resistance of Pengze crucian carp (Carassius auratus var. Pengze)

Shaodan Wang; Shaolin Xie; Aiguo Zhou; Chaonan Zhang; Liufa Wen; Guohuan Xu; Jixing Zou

doi:10.1016/j.fsi.2021.04.017

Effects of mixed antimicrobial peptide on the growth performance, antioxidant and immune responses and disease resistance of Pengze crucian carp (Carassius auratus var. Pengze)

Fish Shellfish Immunol. 2021 Jul:114:112-118. doi: 10.1016/j.fsi.2021.04.017. Epub 2021 Apr 24.

Authors

Shaodan Wang¹, Shaolin Xie², Aiguo Zhou³, Chaonan Zhang⁴, Liufa Wen⁵, Guohuan Xu⁶, Jixing Zou⁷

Affiliations

¹ Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: 20201067007@scau.edu.cn.
² Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: xieshaolinscau@163.com.
³ Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: scauzhouaiguo@163.com.
⁴ Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: zhangchaonan@stu.scau.edu.cn.
⁵ Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, China. Electronic address: wenliufa@126.com.
⁶ State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China. Electronic address: xghfish@163.com.
⁷ Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: zoujixing@scau.edu.cn.

PMID: 33905842
DOI: 10.1016/j.fsi.2021.04.017

Abstract

Antimicrobial peptides have broad-spectrum antibacterial properties and low drug resistance, and they demonstrate great potential as antibiotic substitutes. In this study, five dietary mixed antimicrobial peptide supplement groups were set and fed to Pengze crucian carp for 10 weeks. The 6 groups were G0 (control group) and 5 additional groups: G1 (100 mg/kg), G2 (200 mg/kg), G3 (400 mg/kg), G4 (800 mg/kg) and G5 (1600 mg/kg). The results showed that the final body weight (FBW), weight gain rate (WGR) and specific growth rate (SGR) of fish in G1 and G2 were higher than those of fish in the control group, and G1 was significantly higher than G0 (P < 0.05). In addition, the FBW, WGR, and SGR of the G3 group were significantly lower than those of the G0 group. The chymotrypsin, lipase and amylase activities of G1 and G2 were significantly upregulated compared with G0 and reached peak values in G1. The activity of T-AOC and SOD in the addition group was higher (except G2 and G4) than that in the control groups, and significantly increased in G3 compared to the control group. The activity of MDA in the addition group was lower than that in the control group (p > 0.05). The expression levels of TLR-4, MYD88 and TNF-α in the three organs of the addition group were higher than those in G0 and reached the peak value in G3 (p < 0.05). Furthermore, the expression levels of TLR-4, MYD88 and TNF-α in the three organs of G3 were significantly lower than those in G0 and lower than those in the other supplemented groups. The expression levels of IL-10 and IL-11 tended to be upregulated after A. hydrophila challenge, and G3 in different organs was significantly higher than that in other supplemented groups and G0. The results of this study show that an appropriate amount of mixed antimicrobial peptides can improve the growth performance and antioxidant and immune capabilities of Pengze crucian carp and can also play a positive role in the treatment of A. hydrophila infection.

Keywords: A. hydrophila; Antioxidant; Digestion; Growth; Immunity; Mixed antimicrobial peptide; Pengze crucian carp.

Publication types

Randomized Controlled Trial, Veterinary

MeSH terms

Animal Feed / analysis
Animals
Antioxidants / metabolism*
Diet / veterinary
Gene Expression Regulation / drug effects
Goldfish / growth & development*
Goldfish / immunology
Goldfish / metabolism
Pore Forming Cytotoxic Proteins / administration & dosage
Pore Forming Cytotoxic Proteins / chemistry
Pore Forming Cytotoxic Proteins / pharmacology*

Substances

Antioxidants
Pore Forming Cytotoxic Proteins