The main bottleneck for implementing magnetic nanowires (MNWs) in cell-biology research for multimodal therapeutics is the inapplicability of the current state of the art for selective detection and stimulation of MNWs. Here, we introduce a methodology for selective detection of MNWs in platforms that have multiple magnetic signals, such as future multimodal therapeutics. After characterizing the signatures of MNWs, MNWs were surface-functionalized and internalized into canine osteosarcoma (OSCA-8) cancer cells for cell labeling, manipulation, and separation. We also prepared and characterized magnetic biopolymers as multimodal platforms for future use in controlling the movement, growth, and division of cancer cells. First, it is important to have methods for distinguishing the magnetic signature of the biopolymer from the magnetically labeled cells. For this purpose, we use the projection method to selectively detect and demultiplex the magnetic signatures of MNWs inside cells from those inside magnetic biopolymers. We show that tailoring the irreversible switching field of MNWs by tuning their coercivity is a highly effective approach for generating distinct magnetic biolabels for selective detection of cancer cells. These findings open up new possibilities for selective stimulation of MNWs in multimodal therapeutic platforms for drug delivery, hyperthermia cancer therapy, and mitigating cancer cell movement and proliferation.
Keywords: cell labeling; demultiplexing; magnetic biopolymer; magnetic nanowires; selective detection.