Expression patterns of peroxiredoxin genes in bronchial epithelial cells exposed to diesel exhaust particles

Robson Seriani; Carla Peres de Paula; Anderson Ferreira da Cunha; Marcos Antonio de Oliveira; Paloma Gava Krempel; Daniela Perroni Frias; Elnara Marcia Negri; Thais Mauad; Mariangela Macchione

doi:10.1016/j.yexmp.2021.104641

Expression patterns of peroxiredoxin genes in bronchial epithelial cells exposed to diesel exhaust particles

Exp Mol Pathol. 2021 Jun:120:104641. doi: 10.1016/j.yexmp.2021.104641. Epub 2021 Apr 23.

Authors

Robson Seriani¹, Carla Peres de Paula², Anderson Ferreira da Cunha², Marcos Antonio de Oliveira³, Paloma Gava Krempel⁴, Daniela Perroni Frias⁵, Elnara Marcia Negri⁵, Thais Mauad⁵, Mariangela Macchione⁵

Affiliations

¹ Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, FMUSP- Av. Dr. Arnaldo 455, 1°floor, room 1150, São Paulo, SP 01246-903, Brazil; PROSCED - Consulting, Training and Development, Rua Alto Belo, 742, room 01, São Paulo, SP 03478-040, Brazil. Electronic address: prof.robsonseriani@gmail.com.
² Genetic and Evolution Department, Federal University of São Carlos, UFSCar- Av. Washington Luiz Km 235, São Carlos, SP 13565-905, Brazil.
³ Biosciences Institute, Coastal Campus, São Paulo State University, Structural and Functional Molecular Biology, UNESP- Praça Infante Dom Henrique s/n°, São Vicente, SP 11330-900, Brazil.
⁴ Laboratory for Investigations in Ophthalmology (LIM-33), University of São Paulo Medical School São Paulo, FMUSP - Dr. Arnaldo 455, 5° floorr, São Paulo, SP 01246-903, Brazil.
⁵ Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, FMUSP- Av. Dr. Arnaldo 455, 1°floor, room 1150, São Paulo, SP 01246-903, Brazil.

PMID: 33901418
DOI: 10.1016/j.yexmp.2021.104641

Abstract

Several mechanisms have been suggested to explain the adverse effects of air pollutants on airway cells. One such explanation is the presence of high concentrations of oxidants and pro-oxidants in environmental pollutants. All animal and plant cells have developed several mechanisms to prevent damage by oxidative molecules. Among these, the peroxiredoxins (PRDXs) are of interest due to a high reactivity with reactive oxygen species (ROS) through the functioning of the thioredoxin/thioredoxin reductase system. This study aimed to verify the gene expression patterns of the PRDX family in bronchial epithelial airway cells (BEAS-2B) cells exposed to diesel exhaust particles (DEPs) at a concentration of 15 μg/mL for 1 or 2 h because this it is a major component of particulate matter in the atmosphere. There was a significant decrease in mRNA fold changes of PRDX2 (0.43 ± 0.34; *p = 0.0220), PRDX5 (0.43 ± 0.34; *p = 0.0220), and PRDX6 (0.33 ± 0.25; *p = 0.0069) after 1 h of exposure to DEPs. The reduction in mRNA levels may consequently lead to a decrease in the levels of PRDX proteins, increasing oxidative stress in bronchial epithelial cells BEAS-2B and thus, negatively affecting cellular functions.

Keywords: Air pollution; BEAS-2B; Diesel exhaust particles; Oxidative stress; Peroxiredoxins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bronchi / drug effects
Bronchi / metabolism*
Bronchi / pathology
Cells, Cultured
Epithelial Cells / drug effects
Epithelial Cells / metabolism*
Epithelial Cells / pathology
Gene Expression Regulation*
Humans
Particulate Matter / adverse effects*
Peroxiredoxins / genetics
Peroxiredoxins / metabolism*
Vehicle Emissions / analysis*

Substances

Particulate Matter
Vehicle Emissions
Peroxiredoxins