Potential Applications of Endo-β- N-Acetylglucosaminidases From Bifidobacterium longum Subspecies infantis in Designing Value-Added, Next-Generation Infant Formulas

Hatice Duman; Merve Kaplan; Ayşenur Arslan; Arif Sercan Sahutoglu; Haci Mehmet Kayili; Steven A Frese; Sercan Karav

doi:10.3389/fnut.2021.646275

Potential Applications of Endo-β- N-Acetylglucosaminidases From Bifidobacterium longum Subspecies infantis in Designing Value-Added, Next-Generation Infant Formulas

Front Nutr. 2021 Apr 9:8:646275. doi: 10.3389/fnut.2021.646275. eCollection 2021.

Authors

Hatice Duman¹, Merve Kaplan¹, Ayşenur Arslan¹, Arif Sercan Sahutoglu², Haci Mehmet Kayili³, Steven A Frese^{4

5}, Sercan Karav¹

Affiliations

¹ Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
² Department of Chemistry, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
³ Department of Biomedical Engineering, Karabuk University, Karabük, Turkey.
⁴ Department of Nutrition, University of Nevada, Reno, NV, United States.
⁵ Department of Food Science and Technology, University of Nebraska Lincoln, Lincoln, NE, United States.

Abstract

Human milk is the optimal source of infant nutrition. Among many other health benefits, human milk can stimulate the development of a Bifidobacterium-rich microbiome through human milk oligosaccharides (HMOs). In recent years, the development of novel formulas has placed particular focus on incorporating some of the beneficial functional properties of human milk. These include adding specific glycans aimed to selectively stimulate the growth of Bifidobacterium. However, the bifidogenicity of human milk remains unparalleled. Dietary N-glycans are carbohydrate structures conjugated to a wide variety of glycoproteins. These glycans have a remarkable structural similarity to HMOs and, when released, show a strong bifidogenic effect. This review discusses the biocatalytic potential of the endo-β-N-acetylglucosaminidase enzyme (EndoBI-1) from Bifidobacterium longum subspecies infantis (B. infantis), in releasing N-glycans inherently present in infant formula as means to increase the bifidogenicity of infant formula. Finally, the potential implications for protein deglycosylation with EndoBI-1 in the development of value added, next-generation formulas are discussed from a technical perspective.

Keywords: N-glycans; bifidobacteria; endo-β-N-acetylglucosaminidase; human milk oligosaccharides; infant formula.

Publication types

Review