Multicolor single-molecule FRET for DNA and RNA processes

Xinyu A Feng; Matthew F Poyton; Taekjip Ha

doi:10.1016/j.sbi.2021.03.005

Multicolor single-molecule FRET for DNA and RNA processes

Curr Opin Struct Biol. 2021 Oct:70:26-33. doi: 10.1016/j.sbi.2021.03.005. Epub 2021 Apr 21.

Authors

Xinyu A Feng¹, Matthew F Poyton², Taekjip Ha³

Affiliations

¹ Department of Biology, Johns Hopkins University, Baltimore, MD, USA; Department of Biophysics, Johns Hopkins University, Baltimore, MD, USA.
² Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD, USA.
³ Department of Biophysics, Johns Hopkins University, Baltimore, MD, USA; Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD, USA; Howard Hughes Medical Institute, Baltimore, MD, USA. Electronic address: tjha@jhu.edu.

Abstract

Single-molecule fluorescence resonance energy transfer (smFRET) is a useful tool for observing the dynamics of protein-nucleic acid interactions. Although most smFRET measurements have used two fluorophores, multicolor smFRET measurements using more than two fluorophores offer more information about how protein-nucleic acid complexes dynamically move, assemble, and disassemble. Multicolor smFRET experiments include three or more fluorophores and at least one donor-acceptor pair. This review highlights how multicolor smFRET is being used to probe the dynamics of three different classes of biochemical processes-protein-DNA interactions, chromatin remodeling, and protein translation.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

DNA
Fluorescence Resonance Energy Transfer*
Fluorescent Dyes
Nanotechnology
RNA*

Substances

Fluorescent Dyes
RNA
DNA

Abstract

Publication types

MeSH terms

Substances

Grants and funding