Characterization of lanthanoid-binding proteins using NMR spectroscopy

Abstract

The variety of magnetic properties exhibited by paramagnetic lanthanoids provides outstanding information in NMR-based structural biology and therefore can be a very useful tool for characterizing lanthanoid-binding proteins. Because of their dependence on the relative positions of the protein nuclei and of the lanthanoid ion, the paramagnetic restraints (PCS, PRDC and PRE) provide information on structure and dynamics of proteins. In this Chapter, we cover the use of lanthanoids in structural biology including protein sample preparation, NMR experiments and data interpretation.

Keywords: EF-hands; Gadolinium; Lanthanide; Magnetic susceptibility; Paramagnetic restraints; Pseudocontact shifts; Relaxation.