Next-Generation Sequencing Reveals That Oxidative Phosphorylation Might Be a Key Pathway Differently Expressed in the Third and Fourth Stages Larvae of Angiostrongylus cantonensis

Yue Guo; Hong Chang Zhou; Ying Dong; Hai Yan Dong; Yun Liang Yao; Jing Qian; Hui Zhang; Xiao Yu Li; Zhong Shan Zhang; Han Bing Lin; Tian Zhou; Meng Jia Zhao; Tang Qin Ji; Run Ze Wang; Feng Ping Zhang

doi:10.18502/ijpa.v15i4.4869

Next-Generation Sequencing Reveals That Oxidative Phosphorylation Might Be a Key Pathway Differently Expressed in the Third and Fourth Stages Larvae of Angiostrongylus cantonensis

Iran J Parasitol. 2020 Oct-Dec;15(4):587-595. doi: 10.18502/ijpa.v15i4.4869.

Authors

Yue Guo^{1

2}, Hong Chang Zhou^{1

2}, Ying Dong¹, Hai Yan Dong^{1

2}, Yun Liang Yao^{1

2}, Jing Qian^{1

2}, Hui Zhang¹, Xiao Yu Li¹, Zhong Shan Zhang², Han Bing Lin¹, Tian Zhou¹, Meng Jia Zhao¹, Tang Qin Ji¹, Run Ze Wang¹, Feng Ping Zhang¹

Affiliations

¹ School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China.
² Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, Zhejiang, China.

Abstract

Background: When Angiostrongylus cantonensis develops from the third and fourth stage, it needs to change its host from the middle host, snail to the final host, rat. However, the mechanism involved in this change remains unclear.

Methods: The transcriptome differences of the third and fourth stages of A. cantonensis were explored by next-generation Illumina Hiseq/Miseq sequencing in China, in 2018.

Results: Overall, 137 956 488 clean reads and 20 406 213 373 clean bases of the two stages larvae were produced. Based on the queries against the Gene Ontology (GO), NCBI non-redundant protein sequences (Nr), Swissprot, and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, 14 204 differentially expressed genes (DEGs) were predicted. GO enrichment analysis revealed 5660 DEGs with the top s categories as followings: biological process (GO:0008150, related to 5345 DEGs), cellular component (GO:0005575, related to 5297 DEGs), molecular function (GO:0003674, related to 5290 DEGs). In KEGG enrichment analysis, 116 genes were related to oxidative phosphorylation and 49 genes involved in the glycolytic process.

Conclusion: Metabolism changes, especially oxidative phosphorylation and glycolysis, might play a key role in A. cantonensis infection of its final rat host. Many other pathways might also contribute to the transcriptome changes between these two life stages. Overall, additional studies are needed for further details.

Keywords: Angiostrongylus cantonensis; Fourth stage larvae; Next-generation sequencing; Third stage larvae; Transcriptional sequencing.