Spectrofluorimetric analyzes of thiamine and riboflavin in monofloral honey varieties of africanized bees (Apis mellifera)

Jorge Ederson G Santana; Henrique Douglas M Coutinho; José Galberto M da Costa; Jorge Marcell C Menezes; Hênio do Nascimento Melo Junior; Victor Feitosa Teixeira; Raimundo Nonato Pereira Teixeira

doi:10.1016/j.foodchem.2021.129756

Spectrofluorimetric analyzes of thiamine and riboflavin in monofloral honey varieties of africanized bees (Apis mellifera)

Food Chem. 2021 Apr 9:357:129756. doi: 10.1016/j.foodchem.2021.129756. Online ahead of print.

Authors

Jorge Ederson G Santana¹, Henrique Douglas M Coutinho², José Galberto M da Costa¹, Jorge Marcell C Menezes³, Hênio do Nascimento Melo Junior¹, Victor Feitosa Teixeira¹, Raimundo Nonato Pereira Teixeira¹

Affiliations

¹ Department of Biological Chemistry, Regional University of Cariri, R. Cel. Antonio Luis 1161, 63105000 Crato, CE, Brazil.
² Science and Technology Center, Federal University of Cariri, Av. Tem Raimundo Rocha 1639, 63048080 Juazeiro do Norte, CE, Brazil. Electronic address: hdmcoutinho@urca.br.
³ Science and Technology Center, Federal University of Cariri, Av. Tem Raimundo Rocha 1639, 63048080 Juazeiro do Norte, CE, Brazil.

PMID: 33878583
DOI: 10.1016/j.foodchem.2021.129756

Abstract

This research aimed to study the validation of a viable and low-cost spectrofluorimetric method, capable of detecting and quantifying B vitamins in floral varieties of bee honey. The analyzes were performed using chemical and operational methods of pre-defined solutions and standards, performed in triplicate and the results expressed as mean ± standard deviation. The data were recorded in a Microsoft Office Excel spreadsheet (version 2013) and studied using the statistical program Bioestatic version 3.0. The calibration curves of the vitamins showed the linearity and precision of the method. Subsequently, a descriptive analysis of the data was performed, being considered normal in the Lilliefors normality tests. Finally, the data were subjected to Pearson's correlation test, showing positive and strong correlations, with r (Pearson) ranging from 0.7619 to 1. Honey had positive results regarding the detection and quantification of vitamins B1 and B2 through the spectrofluorimetric method.

Keywords: Analytical validation; Hydrosoluble vitamins and honey; Spectrofluorimetry.