Capturing protein droplets: label-free visualization and detection of protein liquid-liquid phase separation with an aggregation-induced emission fluorogen

Chem Commun (Camb). 2021 Apr 21;57(31):3805-3808. doi: 10.1039/d1cc00947h. Epub 2021 Mar 19.

Abstract

We developed a new method for protein droplet visualization by means of a droplet probe (DroProbe) based on an aggregation-induced emission (AIE) fluorogen. A simple method for viscosity comparison of the protein condensed phase based on the lifetime of the DroProbe was also developed.