A small aliquot (10-14 μL) of ZnO nanoparticles dispersed in deionized water was deposited by evaporation to produce a dry residue on the working area of a screen-printed electrode. An electrochemical test solution containing K₃Fe(CN)6 and KCl was added to the electrode surface for analysis by electrochemical impendence spectroscopy (EIS). Using this deposition analysis technique, a new relationship between the charge transfer resistance (Rct) and the amount of ZnO nanoparticles has been explored. Based on the trend of increasing Rct value with an increase of ZnO nanoparticles, a quantitative analysis method can be established to determine the mass of nanoparticles (0.01-1.00 μg) deposited from an unknown dispersion. To study the matrix effect, addition of Nafion solution to the aqueous dispersion resulted in a change of the linear range to 0.3-0.5 μg nanoparticles. Addition of methanol (10% by volume) to the aqueous dispersion changes the analysis range to 0.2-0.6 μg nanoparticles, while additional methanol (50% by volume) changes the analysis range to 0.06-1.00 μg nanoparticles. The analytical sensitivity, as indicated by the slope of each standard calibration curve, ranked as: aqueous dispersion > Nafion/aqueous dispersion > 10% methanol/aqueous dispersion > 50% methanol/aqueous dispersion. Altogether these results verify that deionized water is the best dispersion medium for EIS analysis of ZnO nanoparticles.