We established an induced pluripotent stem cell (iPSC) reprogrammed from dermal fibroblasts of a 53-year-old healthy man. Retroviruses expressing Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) were employed to initiate the reprogramming and were silenced in iPSCs. The generated iPSCs were pluripotent as determined by immunostaining and expression of pluripotency markers. They were further induced as embryoid bodies in vitro and teratomas in vivo, reminiscent of their full capacity of differentiating into three germ layers. They are with a normal karyotype and genetically identical to donor fibroblasts. This iPSC line provides excellent cell sources for studying and modeling human-specific physiology.
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