Polysaccharide conjugates from Chin brick tea (Camellia sinensis) improve the physicochemical stability and bioaccessibility of β-carotene in oil-in-water nanoemulsions

Qian Li; Jinglan Shi; Xiaolin Du; David Julian McClements; Xiaoqiang Chen; Mengran Duan; Lu Liu; Jing Li; Yundong Shao; Yong Cheng

doi:10.1016/j.foodchem.2021.129714

Polysaccharide conjugates from Chin brick tea (Camellia sinensis) improve the physicochemical stability and bioaccessibility of β-carotene in oil-in-water nanoemulsions

Food Chem. 2021 Apr 1:357:129714. doi: 10.1016/j.foodchem.2021.129714. Online ahead of print.

Authors

Qian Li¹, Jinglan Shi², Xiaolin Du², David Julian McClements³, Xiaoqiang Chen⁴, Mengran Duan², Lu Liu², Jing Li², Yundong Shao⁵, Yong Cheng⁵

Affiliations

¹ National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, College of Bioengineering and Food, Hubei University of Technology, Wuhan, Hubei 430068, China. Electronic address: lqh198299@126.com.
² National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, College of Bioengineering and Food, Hubei University of Technology, Wuhan, Hubei 430068, China.
³ Department of Food Science, University of Massachusetts Amherst, Amherst, MA 01003, USA.
⁴ National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, College of Bioengineering and Food, Hubei University of Technology, Wuhan, Hubei 430068, China. Electronic address: biomed528@163.com.
⁵ Zhejiang Skyherb Biotechnology Inc., Anji, Zhejiang 313300, China.

PMID: 33865002
DOI: 10.1016/j.foodchem.2021.129714

Abstract

A natural antioxidant emulsifier, tea polysaccharide conjugate (TPC), was isolated from Chin brick tea. The impact of TPC on β-carotene stability and bioaccessibility in oil-in-water nanoemulsions was assessed. TPC exhibited strong antioxidant activity and could be used to fabricate stable nanoemulsions (d < 140 nm). The extent of lipid digestion was considerably lower for lipid droplets coated by TPC (68%) than Tween 80 (94%) or whey protein isolate (WPI) (89%), probably because TPC formed interfacial layers that hindered the access of lipases to lipids. The chemical stability of β-carotene in TPC-nanoemulsions was markedly higher than in those formulated with Tween 80 or WPI due to the high antioxidant activity of TPC. The bioaccessibility of β-carotene (20-30%) was independent of emulsifier type. TPC from Chin brick tea can therefore be used as a dual-purpose functional ingredient in emulsified foods.

Keywords: Antioxidant emulsifier; Carotenoids; Chin brick tea; Nanoemulsions; Tea polysaccharide conjugates.