Fabrication of polydopamine-based NIR-light responsive imprinted nanofibrous membrane for effective lysozyme extraction and controlled release from chicken egg white

Noha Amaly; Ahmed Y El-Moghazy; Gang Sun

doi:10.1016/j.foodchem.2021.129613

Fabrication of polydopamine-based NIR-light responsive imprinted nanofibrous membrane for effective lysozyme extraction and controlled release from chicken egg white

Food Chem. 2021 Apr 1:357:129613. doi: 10.1016/j.foodchem.2021.129613. Online ahead of print.

Authors

Noha Amaly¹, Ahmed Y El-Moghazy², Gang Sun³

Affiliations

¹ Department of Biological and Agricultural Engineering, University of California, Davis, CA 95616, USA; Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, Egypt. Electronic address: namaly@ad3.ucdavis.edu.
² Department of Biological and Agricultural Engineering, University of California, Davis, CA 95616, USA; Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, Egypt.
³ Department of Biological and Agricultural Engineering, University of California, Davis, CA 95616, USA. Electronic address: gysun@ucdavis.edu.

PMID: 33864996
DOI: 10.1016/j.foodchem.2021.129613

Abstract

The development of highly efficient performance matrix for protein adsorption and scalable throughput adsorbent is highly desired, especially in pharmaceuticals and food industries. In this work, we present a simple methodology to prepare a nanofibrous membrane based surface molecular imprinted matrix (MIP) for selective separation of lysozyme. The MIP was developed by coating carboxylated poly (vinyl alcohol-co-ethylene) nanofibrous mat (EVOH-CCA NFM) with a near infrared (NIR)-light responsive polydopamine (PDA) layer. The open porous nanofibrous structure and a thin PDA layer endowed the MIPs with adsorption capacity (500 mg.g^-1) within 150 min. The developed surface MIPs not only showed imprinting factor (IF = 4) with reusability upon 5 cycles, but also capability of extracting lysozyme from egg-white directly. The MIPs showed controlled release of extracted lysozyme triggered by the NIR-light responsive property of the PDA layer. Moreover, the released lysozyme possesses good bioactivity, evidenced by efficient decomposition of micrococcus bacterial cell wall.

Keywords: Lysozyme imprinting; NIR-responsive molecular imprinted NFM; Nanofibrous mat; Polydopamine.