DyeCycle Violet efflux, caused by ATP-binding cassette transporters activity, was analyzed in human colorectal adenocarcinoma cell lines SW480, HT-29, Caco-2 by neans of FACSAria III flow cytometer and ImageStreamX Mk II imaging flow cytometer. Along with similarity of cytometry data obtained on the two instruments, the use of imaging flow cytometry made it possible to characterize the morphology of side population cells, as well as morphology of other cell populations differing in the degree of dye accumulation. The population of cells, which are smaller than the side population cells and practically do not take the dye, is of the special interest. Probably, this population may contribute to the tumor resistance to chemotherapy.
Na kletochnykh liniiakh kolorektal'noĭ adenokartsinomy cheloveka SW480, HT-29, Caco-2 proanalizirovana ékskliuziia vital'nogo krasitelia DyeCycle Violet, obuslovlennaia aktivnost'iu ATP-zavisimykh transporterov. Pri skhodstve tsitometricheskikh dannykh, poluchennykh s pomoshch'iu protochnogo tsitometra FACSAria III i protochnogo tsitometra s mikroskopieĭ vysokogo razresheniia ImageStreamX Mk II, primenenie protochnoĭ tsitometrii s funktsieĭ vizualizatsii pozvolilo okharakterizovat' morfologiiu kletok “bokovoĭ populiatsii”, a takzhe drugikh populiatsiĭ kletok, otlichaiushchikhsia po stepeni nakopleniia krasitelia. Osobyĭ interes vyzyvaet populiatsiia kletok bolee melkikh, chem kletki “bokovoĭ populiatsii”, kotorye prakticheski ne okrashivaiutsia krasitelem. Ne iskliucheno, chto éta populiatsiia mozhet vnosit' svoĭ vklad v ustoĭchivost' opukholi k khimioterapii.
Keywords: DyeCycle Violet; colorectal adenocarcinoma; imaging flow cytometry; side population.