Stimulation of Codonopsis pilosula Polysaccharide on Bifidobacterium of Human Gut Bacteria In Vitro

Jiankuan Li; Lina Dong; Yue Liu; Jianping Gao

doi:10.1155/2021/9524913

Stimulation of Codonopsis pilosula Polysaccharide on Bifidobacterium of Human Gut Bacteria In Vitro

Evid Based Complement Alternat Med. 2021 Mar 29:2021:9524913. doi: 10.1155/2021/9524913. eCollection 2021.

Authors

Jiankuan Li¹, Lina Dong², Yue Liu³, Jianping Gao¹

Affiliations

¹ School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, China.
² Central Laboratory, Shanxi Provincial People's Hospital, Affiliate of Shanxi Medical University, Taiyuan 030012, China.
³ China Institutes of Biomedical Sciences, Shanxi University, Taiyuan 030006, China.

Abstract

Objective: To evaluate the prebiotic effects of Codonopsis pilosula polysaccharide (CPP) on human gut bacteria in vitro.

Methods: Codonopsis Radix was extracted with water at 100°C, and the extract was precipitated by 80% ethanol to obtain CPP. Human fresh fecal samples were collected from three healthy adults and used to ferment CPP. The fermented samples were collected to be analyzed by 16S rRNA sequencing.

Results: The results showed that CPP exhibited significantly the stimulation on the growth of genus Bifidobacterium of human gut bacteria (Padj < 0.05). Although CPP also exhibited regulative trends on the genera including Acidaminococcus, Bilophila, Dorea, and Eggerthella, no significant differences were observed (Padj > 0.05), which was likely associated with the limited samples (n = 3).

Conclusion: CPP has the potential to stimulate the growth of Bifidobacterium of the human gut bacteria and to be benefit to human health.