Disruption of Circadian Transcriptome in Lung by Acute Sleep Deprivation

Yuntao Lu; Bing Liu; Junjie Ma; Shuo Yang; Ju Huang

doi:10.3389/fgene.2021.664334

Disruption of Circadian Transcriptome in Lung by Acute Sleep Deprivation

Front Genet. 2021 Mar 30:12:664334. doi: 10.3389/fgene.2021.664334. eCollection 2021.

Authors

Yuntao Lu¹, Bing Liu², Junjie Ma³, Shuo Yang², Ju Huang^{2

4}

Affiliations

¹ Department of Pulmonary and Critical Care Medicine, Huadong Hospital, Fudan University, Shanghai, China.
² Center for Brain Science, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
³ CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
⁴ Department of Anatomy and Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Abstract

Inadequate sleep prevails in modern society and it impairs the circadian transcriptome. However, to what extent acute sleep deprivation (SD) has impact on the circadian rhythms of peripheral tissues is not clear. Here, we show that in mouse lung, a 10-h acute sleep deprivation can alter the circadian expression of approximately 3,000 genes. We found that circadian rhythm disappears in genes related to metabolism and signaling pathways regulating protein phosphorylation after acute sleep deprivation, while the core circadian regulators do not change much in rhythmicity. Importantly, the strong positive correlation between mean expression and amplitude (E-A correlation) of cycling genes has been validated in both control and sleep deprivation conditions, supporting the energetic cost optimization model of circadian gene expression. Thus, we reveal that acute sleep deprivation leads to a profound change in the circadian gene transcription that influences the biological functions in lung.

Keywords: circadian clock; circadian transcriptome; energetic cost; lung; sleep deprivation.