Draft Chromosome Sequences of a Clinical Isolate of the Free-Living Ameba Naegleria fowleri

Ibne Karim M Ali; Alyssa Kelley; Sandeep J Joseph; Subin Park; Shantanu Roy; Jonathan Jackson; Jennifer R Cope; Lori A Rowe; Mark Burroughs; Mili Sheth; Dhwani Batra; Vladimir Loparev

doi:10.1128/MRA.01034-20

Draft Chromosome Sequences of a Clinical Isolate of the Free-Living Ameba Naegleria fowleri

Microbiol Resour Announc. 2021 Apr 15;10(15):e01034-20. doi: 10.1128/MRA.01034-20.

Authors

Ibne Karim M Ali^#¹, Alyssa Kelley^#^{2

3}, Sandeep J Joseph², Subin Park^{2

4}, Shantanu Roy², Jonathan Jackson^{2

5}, Jennifer R Cope², Lori A Rowe⁶, Mark Burroughs⁶, Mili Sheth⁶, Dhwani Batra⁶, Vladimir Loparev⁶

Affiliations

¹ Waterborne Disease Prevention Branch, DFWED/NCEZID, Centers for Disease Control and Prevention, Atlanta, Georgia, USA iali@cdc.gov.
² Waterborne Disease Prevention Branch, DFWED/NCEZID, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
³ ASRT, Inc., Smyrna, Georgia, USA.
⁴ Eagle Medical Services, Atlanta, Georgia, USA.
⁵ IHRC, Inc., Atlanta, Georgia, USA.
⁶ Biotechnology Core Facility Branch, DSR/NCEZID, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

^# Contributed equally.

Abstract

We present the chromosome sequences of a Naegleria fowleri isolate from a human primary amebic meningoencephalitis (PAM) case. The genome sequences were assembled from Illumina HiSeq and PacBio sequencing data and verified with the optical mapping data. This led to the identification of 37 contigs representing 37 chromosomes in N. fowleri.