Bacteria compete with hematite nanoparticles during their uptake by the ciliate Tetrahymena thermophila

Abstract

Bacterial accumulation of engineered nanoparticles (NPs) result in their transfer along the food chain. However, there are a lot of NPs not associated with bacteria. Whether bacteria, as representative biotic particles, influence the biological uptake of these non-associated NPs in aquatic ecosystems is unclear. In the present study, we examined the effects of four bacterial species on the uptake kinetics of polyacrylate-coated hematite nanoparticles (HemNPs) by the ciliate Tetrahymena thermophila. The HemNPs were well dispersed. Their adsorption on the bacteria was low with negligible uptake by T. thermophila through bacterial ingestion. This result demonstrated the feasibility of examining the effects of bacteria on the uptake of non-associated HemNPs. Our study further showed that all four bacterial species inhibited the uptake of HemNPs by T. thermophila; however, the effects of the bacterial cells on the physiological activities of the ciliate with respect to its uptake of HemNPs were negligible. In the absence of phagocytosis by T. thermophila, none of the bacteria inhibited HemNP uptake. This observation suggested that bacterial cells competed with the HemNPs for uptake via phagocytosis. Therefore, in evaluations of the environmental risks of NPs, their competition with biotic particles should be taken into account.

Keywords: Bacteria; Ciliate; Hematite nanoparticles; Phagocytosis; Uptake competition.