Gene expression profiling of early Parkinson's disease patient reveals redox homeostasis

Liwen Zhang; Yijing He; Kecheng Lei; Zhuo Fang; Qian Li; Jun Su; Zhiyu Nie; Yichun Xu; Lingjing Jin

doi:10.1016/j.neulet.2021.135893

Gene expression profiling of early Parkinson's disease patient reveals redox homeostasis

Neurosci Lett. 2021 May 14:753:135893. doi: 10.1016/j.neulet.2021.135893. Epub 2021 Apr 12.

Authors

Liwen Zhang¹, Yijing He², Kecheng Lei³, Zhuo Fang⁴, Qian Li⁵, Jun Su⁵, Zhiyu Nie⁶, Yichun Xu⁷, Lingjing Jin⁸

Affiliations

¹ Neurotoxin Research Center of Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Neurological Department of Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, PR China; National Engineering Research Center for Biochip at Shanghai, Shanghai Biochip Limited Corporation, 201203, Shanghai, PR China; Department of Data & Analytics, WuXi Diagnostics Limited Corporation, 200131, Shanghai, PR China.
² Neurotoxin Research Center of Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Neurological Department of Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, PR China.
³ Neurotoxin Research Center of Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Neurological Department of Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, PR China; Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 30322, Atlanta, GA, USA.
⁴ Department of Data & Analytics, WuXi Diagnostics Limited Corporation, 200131, Shanghai, PR China.
⁵ National Engineering Research Center for Biochip at Shanghai, Shanghai Biochip Limited Corporation, 201203, Shanghai, PR China; Department of Pathology, Shanghai Tongji Hospital, Tongji Hospital Affiliated to Tongji University, 200065, Shanghai, PR China.
⁶ Neurotoxin Research Center of Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Neurological Department of Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, PR China. Electronic address: nzhiyu2002@sina.com.
⁷ National Engineering Research Center for Biochip at Shanghai, Shanghai Biochip Limited Corporation, 201203, Shanghai, PR China; Department of Pathology, Shanghai Tongji Hospital, Tongji Hospital Affiliated to Tongji University, 200065, Shanghai, PR China. Electronic address: yichun_xu@shbiochip.com.
⁸ Neurotoxin Research Center of Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Neurological Department of Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, PR China. Electronic address: lingjingjin@163.com.

PMID: 33857551
DOI: 10.1016/j.neulet.2021.135893

Abstract

Parkinson's disease (PD) is slowly progressive. Due to the lack of specific and sensitive biomarkers, the majority of PD patients are in the advanced stages when diagnosed. This study aimed to investigate biomarkers for early PD diagnosis. We first selected differential mRNAs by analysis of a Gene Expression Omnibus (GEO) data set. Next, we performed RNA sequencing to select differential mRNAs. After an integrated analysis of GEO and RNAseq data, we identified the PD early diagnosis biomarkers associated with oxidative stress. By function analysis, cellular response to hormone stimulus and response to the oxygen-containing compound was involved in the top Gene Set Enrichment Analysis (GSEA)s of the two cohorts. Moreover, SOCS7 was included in these GSEAs coincidentally. Further, by analyzing SOCS7 and its physical interactors, we found they mainly participate in immunity and redox homeostasis related processes, which might play a significant role in PD. Thus, our results suggest SOCS7 might be the potential diagnostic marker for PD.

Keywords: Bioinformatic analysis; Oxidative stress; Parkinson’s disease; SOCS7.

Publication types

Observational Study
Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers / analysis
Biomarkers / metabolism
Case-Control Studies
Early Diagnosis*
Female
Gene Expression Profiling
Gene Regulatory Networks
Healthy Volunteers
Humans
Male
Microarray Analysis
Oxidative Stress / genetics
Parkinson Disease / diagnosis*
Parkinson Disease / genetics
Parkinson Disease / metabolism
Parkinson Disease / pathology
Protein Interaction Maps / genetics
Suppressor of Cytokine Signaling Proteins / analysis*
Suppressor of Cytokine Signaling Proteins / metabolism

Substances

Biomarkers
SOCS7 protein, human
Suppressor of Cytokine Signaling Proteins