Detection and Quantification of African Swine Fever Virus in MA-104 Cells

Ayushi Rai; Sarah Pruitt; Elizabeth Ramirez-Medina; Elizabeth A Vuono; Ediane Silva; Lauro Velazquez-Salinas; Consuelo Carrillo; Manuel V Borca; Douglas P Gladue

doi:10.21769/BioProtoc.3955

Detection and Quantification of African Swine Fever Virus in MA-104 Cells

Bio Protoc. 2021 Mar 20;11(6):e3955. doi: 10.21769/BioProtoc.3955.

Authors

Ayushi Rai^{1

2}, Sarah Pruitt^{1

2}, Elizabeth Ramirez-Medina^{1

2}, Elizabeth A Vuono^{1

3}, Ediane Silva^{1

4}, Lauro Velazquez-Salinas^{1

4}, Consuelo Carrillo⁵, Manuel V Borca¹, Douglas P Gladue¹

Affiliations

¹ Agricultural Research Service Plum Island Animal Disease Center, Greenport, NY, USA.
² Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN, USA.
³ Department of Pathobiology and Population Medicine, Mississippi State University, Starkville, USA.
⁴ Department of Anatomy and Physiology, Kansas State University, Manhattan, USA.
⁵ APHIS, USDA, Plum Island Animal Disease Center, Greenport, New York, USA.

Abstract

Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC #CRL-2378.1), a commercially available cell line isolated from African green monkey ( Cercopithecus aethiops ) kidney epithelial cells, supports the detection of ASFV from field samples with a sensitivity comparable to that of primary swine macrophages. Collection of swine blood or lungs is time costing, which is often not readily available in most veterinary diagnostic laboratories. MA-104 cells could thus be used as substitute for primary swine macrophages to save significant lead time by avoiding the production of primary swine macrophages.

Keywords: ASF; ASFV; African swine fever cell line; Diagnostics; MA-104.