Identification of circulating CD31+CD45+ cell populations with the potential to differentiate into erythroid cells

Maria Chiara G Monaco; Dragan Maric; Ombretta Salvucci; Cristina Antonetti Lamorgese Passeri; Patrizia Accorsi; Eugene O Major; Anna Concetta Berardi

doi:10.1186/s13287-021-02311-y

Identification of circulating CD31⁺CD45⁺ cell populations with the potential to differentiate into erythroid cells

Stem Cell Res Ther. 2021 Apr 13;12(1):236. doi: 10.1186/s13287-021-02311-y.

Authors

Maria Chiara G Monaco^#¹, Dragan Maric^#², Ombretta Salvucci^#³, Cristina Antonetti Lamorgese Passeri⁴, Patrizia Accorsi⁵, Eugene O Major⁶, Anna Concetta Berardi⁷

Affiliations

¹ Viral Immunology Section, National Institute of Neurological, Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
² NINDS Flow Cytometry Core Facility, National Institutes of Health, Bethesda, MD, USA.
³ Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
⁴ Department of Haematology, Laboratory of Stem Cells, Transfusion Medicine and Biotechnologies, Santo Spirito Hospital, Pescara, Italy.
⁵ Department of Haematology, Transfusion Medicine and Biotechnologies, Santo Spirito Hospital, 65125, Pescara, Italy.
⁶ Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
⁷ Department of Haematology, Laboratory of Stem Cells, Transfusion Medicine and Biotechnologies, Santo Spirito Hospital, Pescara, Italy. annacberardi@yahoo.it.

^# Contributed equally.

Abstract

Erythro-myeloid progenitors (EMP) are found in a population of cells expressing CD31 and CD45 markers (CD31⁺CD45⁺). A recent study indicated that EMPs persist until adulthood and can be a source of endothelial cells. We identified two sub-populations of EMP cells, CD31^lowCD45^low and CD31^highCD45⁺, from peripheral blood that can differentiate into cells of erythroid lineage. Our novel findings add to the current knowledge of hematopoietic lineage commitment, and our sequential, dual-step, in vitro culture model provides a platform for the study of the molecular and cellular mechanisms underlying human hematopoiesis and erythroid differentiation.

Keywords: CD31+CD45+ circulating cells; Erythro-myeloid progenitors; Human erythropoiesis.

Publication types

Letter
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation
Endothelial Cells*
Erythroid Cells
Hematopoiesis
Hematopoietic System*
Humans