LncRNA PSMG3AS1 promotes proliferation of non-small cell lung cancer cells by sponging miR-613 to upregulate SphK1

Weiyu Shen; Xunyu Hong; Chenghua Jin; Yong Xi

doi:10.1080/15384101.2021.1900499

LncRNA PSMG3AS1 promotes proliferation of non-small cell lung cancer cells by sponging miR-613 to upregulate SphK1

Cell Cycle. 2021 May;20(9):829-838. doi: 10.1080/15384101.2021.1900499. Epub 2021 Apr 14.

Authors

Weiyu Shen¹, Xunyu Hong², Chenghua Jin¹, Yong Xi¹

Affiliations

¹ Department of Thoracic Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo City, Zhejiang Province, P.R. China.
² Department of Internal Medicine, Ningbo Women and Children's Hospital, Ningbo City, Zhejiang Province, P.R. China.

Abstract

PSMG3-AS1 is a characterized oncogenic lncRNA in breast cancer, while its role in other cancers remains unclear. This study was to investigate the role and underlying mechansim of PSMG3-AS1 in non-small cell lung cancer (NSCLC). In this study, we found that PSMG3-AS1 could interact with miR-613. The expression of PSMG3-AS1 was upregulated in NSCLC, while the expression of miR-613 was downregulated in NSCLC. However, PSMG3-AS1 and miR-613 were not significantly correlated with each other. In NSCLC cells, PSMG3-AS1 and miR-613 overexpression failed to regulate the expression of each other. Interestingly, PSMG3-AS1 overexpression led to upregulated SphK1, a downstream target of miR-613. In addition, PSMG3-AS1 overexpression reduced the inhibitory effects of miR-613 on NSCLC cell proliferation. Therefore, PSMG3-AS1 may promote the proliferation of NSCLC cells by sponging miR-613 to upregulate SphK1.

Keywords: Non-small cell lung cancer; miR-613; psmg3-as1; sphk1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Carcinoma, Non-Small-Cell Lung / genetics*
Carcinoma, Non-Small-Cell Lung / pathology*
Cell Line, Tumor
Cell Proliferation / genetics
Gene Expression Regulation, Neoplastic
Humans
Lung Neoplasms / genetics*
Lung Neoplasms / pathology*
MicroRNAs / genetics
MicroRNAs / metabolism*
Phosphotransferases (Alcohol Group Acceptor) / genetics*
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism*
Up-Regulation / genetics*

Substances

MIRN613 microRNA, human
MicroRNAs
RNA, Long Noncoding
Phosphotransferases (Alcohol Group Acceptor)
sphingosine kinase

Grants and funding

We thank the financial support from the major science and technology innovation in 2025 projects of Ningbo, China(Grant No. 2019B10039) and Commission of Ningbo (2018A50).