Applications of western blot technique: From bench to bedside

Biochem Mol Biol Educ. 2021 Jul;49(4):509-517. doi: 10.1002/bmb.21516. Epub 2021 Apr 13.

Abstract

Western blot (WB) or immunoblot is a workhorse method. It is commonly used by biologists for study of different aspects of protein biomolecules. In addition, it has been widely used in disease diagnosis. Despite some limitations such as long time, different applications of WB have not been limited. In the present review, we have summarized scientific and clinical applications of WB. In addition, we described some new generation of WB techniques.

Keywords: antibody; clinical application; protein immunoblot; scientific application; western blot.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Blotting, Western / methods*
  • Blotting, Western / standards*
  • Humans
  • Proteins / analysis*
  • Proteins / isolation & purification
  • Proteins / metabolism*
  • Translational Research, Biomedical*

Substances

  • Proteins