Bacterial diversity in petroleum coke based biofilters treating oil sands process water

Abstract

Adopting nature-based solutions for the bioremediation of oil sands process water (OSPW) is of significant interest, which requires a thorough understanding of how bacterial communities behave within treatment systems operated under natural conditions. This study investigates the OSPW remediation potential of delayed petroleum-coke (PC), which is a byproduct of bitumen upgrading process and is readily available at oil refining sites, in fixed-bed biofilters particularly for the degradation of naphthenic acids (NAs) and aromatics. The biofilters were operated continuously and total and active bacterial communities were studied by DNA and RNA-based amplicon sequencing in a metataxonomic fashion to extrapolate the underlying degradation mechanisms. The results of total community structure indicated a high abundance of aerobic bacteria at all depths of the biofilter, e.g., Porphyrobacter, Legionella, Pseudomonas, Planctomyces. However, redox conditions within the biofilters were anoxic (-153 to -182 mV) that selected anaerobic bacteria to actively participate in the remediation of OSPW, i.e., Ruminicoccus, Eubacterium, Faecalibacterium, Dorea. After 15 days of operation, the removal of classical NAs was recorded up to 20% whereas oxidized NAs species were poorly removed, i.e., O₃-NAs: 4.8%, O₄-NAs: 1.2%, O₅-NAs: 1.7%, and O₆-NAs: 0.5%. Accordingly, monoaromatics, diaromatics, and triaromatics were removed up to 16%, 22%, and 15%, respectively. The physiology of the identified genera suggested that the degradation in the PC-based biofilters was most likely proceeded in a scheme similar to beta-oxidation during anaerobic digestion process. The presence of hydrogenotrophic methanogens namely Methanobrevibacter and Methanomassiliicoccus and quantification of mcrA gene (2.4 × 10² to 8.7 × 10² copies/mg of PC) revealed that methane production was likely occurring in a syntrophic mechanism during the OSPW remediation. A slight reduction in toxicity was also observed. This study suggests that PC-based biofilters may offer some advantages in the remediation of OSPW; however, the production of methane could be of future concerns if operated at field-scale.

Keywords: Anaerobic bacteria; Delayed petroleum coke; Fixed-bed biofiltration; Naphthenic acids; OSPW.