Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu)

Nifei Wang; Yan Wu; Gege Jia; Changlu Wang; Dongguang Xiao; H Douglas Goff; Qingbin Guo

doi:10.1016/j.carbpol.2021.117945

Structural characterization and immunomodulatory activity of mycelium polysaccharide from liquid fermentation of Monascus purpureus (Hong Qu)

Carbohydr Polym. 2021 Jun 15:262:117945. doi: 10.1016/j.carbpol.2021.117945. Epub 2021 Mar 15.

Authors

Nifei Wang¹, Yan Wu², Gege Jia³, Changlu Wang⁴, Dongguang Xiao⁵, H Douglas Goff⁶, Qingbin Guo⁷

Affiliations

¹ State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: wangnifei@tust.edu.cn.
² Shanghai Engineering Research Center of Food Safety, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address: wuy@sjtu.edu.cn.
³ State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: gegejia@mail.tust.edu.cn.
⁴ State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: clw123@tust.edu.cn.
⁵ College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: Xiao99@tust.edu.cn.
⁶ Department of Food Science, University of Guelph, Guelph, Ontario, N1G 2W1, Canada. Electronic address: dgoff@uoguelph.ca.
⁷ State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: guoqingbin008322@tust.edu.cn.

PMID: 33838822
DOI: 10.1016/j.carbpol.2021.117945

Abstract

Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography. By combining monosaccharide composition analysis, methylation analysis and 1D & 2D NMR, the structure of sub-fractions was systematically characterized. Both MPS-1 and MPS-2 were comprised of mannose, glucose and galactose in the molar ratio of 1.5:1.6:1.0 and 10.6:1.0:13.8, respectively. The backbone of them both consisted of 2-α-Manp with several different branched chains. However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2. The proposed structures of MPS-3 and MPS-4 were not obtained due to the fairly complex molecular structure and relatively low yield. Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.

Keywords: Immunomodulatory activity; Liquid fermentation; Monascus purpureus; Mycelium polysaccharide; Structural characterization.

MeSH terms

Animals
Cell Proliferation / drug effects
Cytokines / metabolism
Fermentation
Fungal Polysaccharides / chemistry*
Fungal Polysaccharides / pharmacology*
Galactose / chemistry
Glucose / chemistry
Immunologic Factors / chemistry*
Immunologic Factors / pharmacology*
Immunomodulation
Magnetic Resonance Spectroscopy / methods
Mannose / chemistry
Methylation
Mice
Molecular Structure
Monascus / chemistry*
Monosaccharides / analysis
Mycelium / chemistry*
RAW 264.7 Cells

Substances

Cytokines
Fungal Polysaccharides
Immunologic Factors
Monosaccharides
Glucose
Mannose
Galactose