miR-1253, a novel tumor suppressor gene in colon cancer, is associated with poor patients prognosis

Abstract

MiR-1253 has been reported to play vital roles in a variety of cancers. However, its function in the progression and prognosis of colon cancer remains unclear. Here, the clinical significance and biological function of miR-1253 in the development of colon cancer was investigated. The expression of miR-1253 was identified in colon cancer tissues and cell lines by qRT-PCR. The prognostic value of miR-1253 was evaluated by Kaplan-Meier and Cox regression analysis. The roles of miR-1253 in regulating cell proliferation, migration, and invasion of colon cancer were evaluated in vitro by CCK8 and Transwell assay. It was found that miR-1253 was significantly downregulated in colon cancer tissues and cell lines (P < 0.001). The downregulation of miR-1253 was associated with the TNM stage (P = 0.007) and lymph node metastasis (P = 0.037) of patients. MiR-1253 (HR factor = 2.564, 95% CI = 1.077-6.107, P = 0.033) and TNM stage (HR factor = 2.899, 95% CI = 1.024-8.205, P = 0.045) were identified as independent factors that can predict the prognosis of patients. Functionally, miR-1253 acts as a tumor suppressor that inhibited cell proliferation, migration, and invasion of colon cancer through targeting EZH2. MiR-1253 was identified as a prognostic biomarker and a tumor suppressor of colon cancer.

Keywords: Colon cancer; Development; MiR-1253; Prognosis.