Morphological and molecular characterization of Heterodera dunensis n. sp. (Nematoda: Heteroderidae) from Gran Canaria, Canary Islands

Phougeishangbam Rolish Singh; Gerrit Karssen; Marjolein Couvreur; Wim Bert

doi:10.21307/jofnem-2020-098

Morphological and molecular characterization of Heterodera dunensis n. sp. (Nematoda: Heteroderidae) from Gran Canaria, Canary Islands

J Nematol. 2020 Nov 24:52:e2020-98. doi: 10.21307/jofnem-2020-098. eCollection 2020.

Authors

Phougeishangbam Rolish Singh^{1

2}, Gerrit Karssen^{1

2}, Marjolein Couvreur¹, Wim Bert¹

Affiliations

¹ Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium.
² National Plant Protection Organization, Wageningen Nematode Collection, P.O. Box 9102, 6700, HC, Wageningen, The Netherlands.

Abstract

Heterodera dunensis n. sp. from the coastal dunes of Gran Canaria, Canary Islands, is described. This new species belongs to the Schachtii group of Heterodera with ambifenestrate fenestration, presence of prominent bullae, and a strong underbridge of cysts. It is characterized by vermiform second-stage juveniles having a slightly offset, dome-shaped labial region with three annuli, four lateral lines, a relatively long stylet (27-31 µm), short tail (35-45 µm), and 46 to 51% of tail as hyaline portion. Males were not found in the type population. Phylogenetic trees inferred from D2-D3 of 28S, partial ITS, and 18S of ribosomal DNA and COI of mitochondrial DNA sequences indicate a position in the 'Schachtii clade'.

Keywords: 18S; 28S; COI; Canary Islands; Cyst nematode; Gran Canaria; Heterodera dunensis; ITS; Plant-parasitic nematodes; Schachtii; Systematics; Taxonomy.