Complete mitochondrial genome of nearly threatened freshwater ornamental fish, Microphis deocata and its phylogenetic relationship within Syngnathidae

Lakshman Sahoo; Ashoktaru Barat; Paramananda Das; Bismay Sahoo; Gargee Das; Jitendra K Sundaray; Sangram K Sahoo; Saroj K Swain; Khuntia Murmu; Dandadhar Sarma

doi:10.1080/23802359.2020.1835572

Complete mitochondrial genome of nearly threatened freshwater ornamental fish, Microphis deocata and its phylogenetic relationship within Syngnathidae

Mitochondrial DNA B Resour. 2021 Mar 26;6(3):1207-1208. doi: 10.1080/23802359.2020.1835572.

Affiliations

¹ Fish Genetics and Biotechnology Division, ICAR - Central Institute of Freshwater Aquaculture, Bhubaneswar, India.
² Department of Zooology, Gauhati University, Guwahati, India.

Abstract

Microphis deocata (deocata pipefish), belonging to family Syngnathidae, is one of the important indigenous ornamental fish species listed as near threatened in the IUCN red list. Here, we first report the complete mitochondrial genome of deocata pipefish using Illumina next-generation sequencing platform. The total length of the mitogenome is 16,526 bp. It encompasses 13 protein coding genes, 2 ribosomal rRNAs, and 22 tRNAs. The WANCY region (a cluster of five tRNA genes) contains the 50 bp O_L light strand origin of replication. Phylogenetic analysis of Syngnathidae revealed M. deocata to cluster with Oostethus manadensis, forming a sister group with Doryrhamphus japonicas and Dunckerocampus dactyliophorus. The mitochondrial genome sequence data generated in the present study will play an important role in population genetic analysis and developing conservation strategies for this species.

Keywords: Deocata; Mitochondrial genome; NGS; Syngnathidae; phylogenetics; protein coding genes.