Colorimetric detection of homocysteine by a pyridylazo dye-based Cu2+ complex via indicator displacement mechanism

Abstract

A BrPAPS based Cu²⁺ complex has been developed as a colorimetric probe for the selective recognition of homocysteine (Hcy) over cysteine (Cys) and glutathione (GSH) in an aqueous solution via the indicator displacement assay. BrPAPS formed a complex with Cu²⁺ in a 1:1 ratio (BrPAPS-Cu²⁺) accompanied by the color change from yellow to red. Detecting Hcy is based on high affinity of Hcy for Cu²⁺. The addition of Hcy to BrPAPS-Cu²⁺ caused the complex formation of Hcy with Cu²⁺ in a 2:1 stoichiometry, resulting a hypsochromic shift with change back of color from red to yellow by the release of BrPAPS from BrPAPS-Cu²⁺. The absorption response is linear with the Hcy concentration in the range of 0-20 μM with a detection limit of 1.46 μM. Moreover, the detection of Hcy was not significantly affected by other amino acids from the competition experiments. Thus, BrPAPS-Cu²⁺ can be used as a simple probe for Hcy in aqueous solution.

Keywords: Chemosensor; Colorimetric detection; Copper ion; Homocysteine; Pyridylazo dye.